論文 - 詳細
| RRC ID | 41443 |
|---|---|
| 著者 | Kanno S, Anuradha CD, Hirano S. |
| タイトル | Chemotactic responses of osteoblastic MC3T3-E1 cells toward zinc chloride. |
| ジャーナル | Biol Trace Elem Res |
| Abstract |
Although zinc (Zn) is known to participate in bone formation, its exact role in the remodeling of this tissue has not been fully clarified. The present study was designed to investigate whether Zn has a role at the resorptive sites in vitro. We investigated the migration of osteoblastic MC3T3-E1 cells in response to Zn using a Boyden chamber assay. Exposure of MC3T3-E1 cells to Zn stimulated the migration of MC3T3-E1 cells. Checkerboard analysis revealed that the migration of MC3T3-E1 cells toward Zn was a directional (chemotaxis) rather than a random (chemokinesis) motion. Pretreatment of MC3T3-E1 cells with pertussis toxin completely blocked the chemotactic response of cells to Zn, indicating that it is mediated by G-protein-coupled receptors. Because the bone is one of the major Zn storage sites, we suggest that Zn released from bone-resorptive sites plays an important role in the recruitment of osteoblasts and bone renewal. |
| 巻・号 | 83(1) |
| ページ | 49-55 |
| 公開日 | 2001-10-1 |
| DOI | 10.1385/BTER:83:1:49 |
| PII | BTER:83:1:49 |
| PMID | 11694002 |
| MeSH | 3T3 Cells Animals Becaplermin Bone Remodeling / drug effects Bone Remodeling / physiology Calcium / pharmacology Chemotaxis / drug effects* Chemotaxis / physiology Chlorides / pharmacology* GTP-Binding Proteins / physiology Mice Osteoblasts / drug effects* Osteoblasts / physiology* Pertussis Toxin Platelet-Derived Growth Factor / pharmacology Proto-Oncogene Proteins c-sis Receptors, Cell Surface / drug effects Receptors, Cell Surface / physiology Virulence Factors, Bordetella / pharmacology Zinc Compounds / pharmacology* |
| IF | 2.639 |
| 引用数 | 10 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) NIH3T3-3(RCB0150) |