論文 - 詳細
| RRC ID | 41455 |
|---|---|
| 著者 | Wu F, Nishioka M, Fujita J, Murota M, Ohtsuki Y, Ishida T, Kuriyama S. |
| タイトル | Expression of cytokeratin 19 in human hepatocellular carcinoma cell lines. |
| ジャーナル | Int J Oncol |
| Abstract |
It has been reported that the cytokeratin 19 (CK19) fragment, CYFRA 21-1, is increased in sera of some hepatocellular carcinoma patients. We hypothesized that CYFRA 21-1 might be released directly from hepatocellular carcinoma. To investigate this mechanism, we evaluated expression of CK19 in 8 human cell lines by immunoradiometric assay, Western blotting, immunohistochemistry, and reverse transcriptase-polymerase chain reaction in Chang liver cells, 2 cholangiocarcinoma cell lines, and 5 hepatocellular carcinoma cell lines. Three of 5 hepatocellular carcinoma cell lines released CYFRA 21-1, synthesized CK19 protein and expressed mRNA for CK19, as observed in 2 cholangiocarcinoma cell lines. In contrast, there was no expression of CK19 in Chang liver cells or 2 of 5 hepatocellular carcinoma cell lines. Analysis of genomic DNA for CK19 demonstrated that exon 1 was not amplified in the 3 cell lines not expressing CK19. However, there were no point mutations within exon 1 and the promoter region of CK19 in hepatocellular carcinoma cell lines. Our present study demonstrates that: i) the expression of CK19 was evident in some human hepatocellular carcinoma cell lines, and ii) the expression of mRNA for CK19 was related to the release of CYFRA 21-1. These results partially clarify the mechanism by which some hepatocellular carcinoma cell lines produce CK19 and others do not. |
| 巻・号 | 20(1) |
| ページ | 31-7 |
| 公開日 | 2002-1-1 |
| PMID | 11743639 |
| MeSH | Antigens, Neoplasm / genetics Antigens, Neoplasm / metabolism* Base Sequence Bile Duct Neoplasms / genetics Bile Duct Neoplasms / metabolism* Blotting, Western Carcinoma, Hepatocellular / genetics Carcinoma, Hepatocellular / metabolism* Cholangiocarcinoma / genetics Cholangiocarcinoma / metabolism* DNA Primers DNA, Neoplasm / analysis Gene Expression Humans Immunoenzyme Techniques Immunoradiometric Assay Keratin-19 Keratins / genetics Keratins / metabolism* Liver Neoplasms / genetics Liver Neoplasms / metabolism* Molecular Sequence Data RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction Tumor Cells, Cultured |
| IF | 3.899 |
| 引用数 | 15 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RBE(RCB1292) |