| RRC ID |
41470
|
| 著者 |
Yamato M, Konno C, Utsumi M, Kikuchi A, Okano T.
|
| タイトル |
Thermally responsive polymer-grafted surfaces facilitate patterned cell seeding and co-culture.
|
| ジャーナル |
Biomaterials
|
| Abstract |
Tissue engineering constructs that effectively duplicate natural tissue function must also maintain tissue architectural and organization features, particularly the integration of multiple cell types preserving distinct, integrated phenotypes. Cell-cell communication and biochemical cross-talk have been shown to be essential for the maintenance of differentiated cell functions in tissues and organs. Current limitations of cell-culture hinder progress in understanding the features and dynamics of heterotypic cell communication pathways critical to developing more sophisticated or effective tissue-engineered devices. We describe a method to conveniently electron-beam pattern cell culture surfaces with thermo-responsive polymer chemistry that exploits changes in cell-polymer adhesive interactions over a temperature window amenable for high-throughput cell culture. Cells seeded on these patterned surfaces at 20 degrees C adhere only to surface areas lacking thermo-responsive grafting chemistry; grafted domains at 20 degrees C are hydrophilic and non-cell adhesive. The culture temperature is then increased to 37 degrees C collapsing the hydrated grafted chemistry. A second cell type is added to the culture and adheres only to these exposed relatively hydrophobic grafted patterns. Both cell types can then be effectively co-cultured at 37 degrees C under multiple conditions. Long-term cell pattern fidelity and differentiated cell functions characteristic of each co-planar cell type are observed. This method is simple and has few limitations, compared with other existing co-culture methods.
|
| 巻・号 |
23(2)
|
| ページ |
561-7
|
| 公開日 |
2002-1-1
|
| DOI |
10.1016/s0142-9612(01)00138-7
|
| PII |
S0142-9612(01)00138-7
|
| PMID |
11761176
|
| MeSH |
Animals
Cell Adhesion
Coculture Techniques
Male
Polymers*
Rats
Rats, Wistar
Surface Properties
Temperature
|
| IF |
10.317
|
| 引用数 |
228
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
|
