論文 - 詳細
| RRC ID | 41512 |
|---|---|
| 著者 | Jiao W, Miyazaki K, Kitajima Y. |
| タイトル | Inverse correlation between E-cadherin and Snail expression in hepatocellular carcinoma cell lines in vitro and in vivo. |
| ジャーナル | Br J Cancer |
| Abstract |
Hepatocellular carcinoma is a well-known malignancy in the world. However, the molecular mechanism of carcinogenesis and tumour progression remains unclear. Recently, reduced E-cadherin expression due to transcriptional suppressor Snail was proven in a panel of epithelial and dedifferentiated cells derived from carcinomas of various etiologies. In the present study, we examined Snail and E-cadherin mRNA/protein expression in five hepatocellular carcinoma cell lines with variable phenotypes (HuL-1, Hep-G(2), Changliver, HLE, and HLF). The results demonstrated that the presence of Snail mRNA in HuL-1, Changliver, HLE and HLF cells detected by RT-PCR, which was further proven by in situ hybridization in tumours induced by HuL-1, Changliver, and HLF cells where Snail mRNA signals expressed in each of the sections. By contrast, E-cadherin mRNA and protein expression were only detected in Hep-G(2) cells by RT-PCR and Western blot, respectively. These results were also consistent with the data obtained from in vivo immunohistochemical staining where membranous expression of endogenous E-cadherin protein was revealed only in tumour sections induced by Hep-G(2) cells. Here we are the first to report that there is an inverse correlation between Snail and E-cadherin expression in HCC cells as well. |
| 巻・号 | 86(1) |
| ページ | 98-101 |
| 公開日 | 2002-1-7 |
| DOI | 10.1038/sj.bjc.6600017 |
| PII | 6600017 |
| PMID | 11857019 |
| PMC | PMC2746537 |
| MeSH | Animals Cadherins / analysis Cadherins / genetics* Carcinoma, Hepatocellular / metabolism* DNA-Binding Proteins / analysis DNA-Binding Proteins / genetics* Humans In Situ Hybridization Liver Neoplasms / metabolism* Male Mice Mice, Inbred BALB C RNA, Messenger / analysis Reverse Transcriptase Polymerase Chain Reaction Snail Family Transcription Factors Transcription Factors / analysis Transcription Factors / genetics* Tumor Cells, Cultured |
| IF | 5.791 |
| 引用数 | 120 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 13 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HuL-1 Hep G2(RCB0459) |