RRC ID 41518
Author Murakami M, Yoshihara K, Shimbara S, Lambeau G, Gelb MH, Singer AG, Sawada M, Inagaki N, Nagai H, Ishihara M, Ishikawa Y, Ishii T, Kudo I.
Title Cellular arachidonate-releasing function and inflammation-associated expression of group IIF secretory phospholipase A2.
Journal J Biol Chem
Abstract Here we report the cellular arachidonate (AA)-releasing function of group IIF secretory phospholipase A(2) (sPLA(2)-IIF), a sPLA(2) enzyme uniquely containing a longer C-terminal extension. sPLA(2)-IIF increased spontaneous and stimulus-dependent release of AA, which was supplied to downstream cyclooxygenases and 5-lipoxygenase for eicosanoid production. sPLA(2)-IIF also enhanced interleukin 1-stimulated expression of cyclooxygenase-2 and microsomal prostaglandin E synthase. AA release by sPLA(2)-IIF was facilitated by oxidative modification of cellular membranes. Cellular actions of sPLA(2)-IIF occurred independently of the heparan sulfate proteoglycan glypican, which acts as a functional adaptor for other group II subfamily sPLA(2)s. Confocal microscopy revealed the location of sPLA(2)-IIF on the plasma membrane. The unique C-terminal extension was crucial for its plasma membrane localization and optimal cellular functions. sPLA(2)-IIF expression was increased in various tissues from lipopolysaccharide-treated mice and in ears of mice with experimental atopic dermatitis. In human rheumatoid arthritic joints, sPLA(2)-IIF was detected in synovial lining cells, capillary endothelial cells, and plasma cells. These results suggest that sPLA(2)-IIF is a potent regulator of AA metabolism and participates in the inflammatory process under certain conditions.
Volume 277(21)
Pages 19145-55
Published 2002-5-24
DOI 10.1074/jbc.M112385200
PII S0021-9258(20)85205-3
PMID 11877435
MeSH Animals Arachidonic Acid / metabolism* Base Sequence Cell Line DNA Primers Dinoprostone / biosynthesis Humans Inflammation / enzymology Inflammation / metabolism* Male Mice Mice, Inbred BALB C Mice, Inbred C57BL Phospholipases A / metabolism* Phospholipases A2 Rats Tumor Cells, Cultured
IF 4.238
Times Cited 57
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Human and Animal Cells RBL 2H3(RCB2782)