| RRC ID |
41518
|
| 著者 |
Murakami M, Yoshihara K, Shimbara S, Lambeau G, Gelb MH, Singer AG, Sawada M, Inagaki N, Nagai H, Ishihara M, Ishikawa Y, Ishii T, Kudo I.
|
| タイトル |
Cellular arachidonate-releasing function and inflammation-associated expression of group IIF secretory phospholipase A2.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
Here we report the cellular arachidonate (AA)-releasing function of group IIF secretory phospholipase A(2) (sPLA(2)-IIF), a sPLA(2) enzyme uniquely containing a longer C-terminal extension. sPLA(2)-IIF increased spontaneous and stimulus-dependent release of AA, which was supplied to downstream cyclooxygenases and 5-lipoxygenase for eicosanoid production. sPLA(2)-IIF also enhanced interleukin 1-stimulated expression of cyclooxygenase-2 and microsomal prostaglandin E synthase. AA release by sPLA(2)-IIF was facilitated by oxidative modification of cellular membranes. Cellular actions of sPLA(2)-IIF occurred independently of the heparan sulfate proteoglycan glypican, which acts as a functional adaptor for other group II subfamily sPLA(2)s. Confocal microscopy revealed the location of sPLA(2)-IIF on the plasma membrane. The unique C-terminal extension was crucial for its plasma membrane localization and optimal cellular functions. sPLA(2)-IIF expression was increased in various tissues from lipopolysaccharide-treated mice and in ears of mice with experimental atopic dermatitis. In human rheumatoid arthritic joints, sPLA(2)-IIF was detected in synovial lining cells, capillary endothelial cells, and plasma cells. These results suggest that sPLA(2)-IIF is a potent regulator of AA metabolism and participates in the inflammatory process under certain conditions.
|
| 巻・号 |
277(21)
|
| ページ |
19145-55
|
| 公開日 |
2002-5-24
|
| DOI |
10.1074/jbc.M112385200
|
| PII |
S0021-9258(20)85205-3
|
| PMID |
11877435
|
| MeSH |
Animals
Arachidonic Acid / metabolism*
Base Sequence
Cell Line
DNA Primers
Dinoprostone / biosynthesis
Humans
Inflammation / enzymology
Inflammation / metabolism*
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Phospholipases A / metabolism*
Phospholipases A2
Rats
Tumor Cells, Cultured
|
| IF |
4.238
|
| 引用数 |
57
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RBL 2H3(RCB2782) |
