RRC ID 41519
Author Jiang B, Hattori N, Liu B, Kitagawa K, Inagaki C.
Title Expression of swelling- and/or pH-regulated chloride channels (ClC-2, 3, 4 and 5) in human leukemic and normal immune cells.
Journal Life Sci.
Abstract Chloride channels on immune cells reportedly play important roles in cell volume regulation, cell proliferation and immune functions, but they are not well characterized at the molecular level. We examined the expression of swelling-and/or pH-regulated chloride channels (ClC-2, 3, 4 and 5) in human leukemic cell lines [Jurkat and Hut-78 (T cells), Raji and Daudi (B cells), K-562 and HL-60 (myeloid cells)] and T cells, B cells and neutrophils from 8 normal subjects to clarify the difference of their expression among different cell types and maturity. Semi-quantitative RT-PCR and Northern blot analysis showed that ClC-3 was most abundantly expressed in all cells regardless of the cell types and maturity, while expression of ClC-2 was weak in these cells. Expression of ClC-4 was observed mainly in leukemic B cell lines, and in B cells and neutrophils from normal subjects. ClC-5 was expressed in all cell lines, while it was observed in only T and B cells but not in neutrophils from normal subjects. Thus, these chloride channels (ClC-2, 3, 4 and 5) showed distinct distribution among human immune cells, suggesting that they have specific roles in these cells. Molecular identification of chloride channels in leukocytes of different types and maturity may provide a new approach for the treatment of leukemia.
Volume 70(12)
Pages 1383-94
Published 2002-2-8
DOI 10.1016/s0024-3205(01)01517-x
PII S0024-3205(01)01517-X
PMID 11883714
MeSH Adult B-Lymphocytes / metabolism Blotting, Northern Cell Separation Chloride Channels / biosynthesis* Chloride Channels / classification Chloride Channels / genetics Humans Jurkat Cells / metabolism Leukocytes / metabolism* Neutrophils / metabolism RNA, Messenger / metabolism RNA, Neoplasm / analysis Reverse Transcriptase Polymerase Chain Reaction T-Lymphocytes / metabolism
IF 3.448
Times Cited 14
WOS Category MEDICINE, RESEARCH & EXPERIMENTAL PHARMACOLOGY & PHARMACY
Resource
Human and Animal Cells