論文 - 詳細
| RRC ID | 41533 |
|---|---|
| 著者 | Zhang Q, Wang C, Babukutty Y, Ohyama T, Kogoma M, Kodama M. |
| タイトル | Biocompatibility evaluation of ePTFE membrane modified with PEG in atmospheric pressure glow discharge. |
| ジャーナル | J Biomed Mater Res |
| Abstract |
ePTFE membranes were modified by poly(ethylene glycol) having a molecular weight of 600 (PEG-600) in atmospheric pressure glow discharge (APG) plasma treatment. ePTFE membranes were immersed in 1%, 3%, or 5% (w/v) PEG-600 in dehydrated ethanol. PEG-600-penetrated ePTFE membranes were dried in a vacuum to immediately remove ethanol, then treated with APG at 20 kHz and 60-70 W for 15 min and thoroughly washed with ethanol and water. PEG-600-modified ePTFE membranes were analyzed using contact angle measurement, Fourier transform infrared attenuated total reflectance (FTIR-ATR), and scanning electron microscopy (SEM). ePTFE membrane contact angles were reduced after PEG-600 plasma treatment. FTIR-ATR spectra showed an absorption band due to a PEG hydroxyl group (-OH). SEM showed that ePTFE fiber surfaces were uniformly immobilized with PEG-600 and retained their porous structure. A general biological evaluation of the PEG-modified ePTFE membranes showed no cytotoxicity on CHO-K1 cell lines and no hemolytic action. Albumin adsorption on the PEG-modified ePTFE membranes increased with increasing PEG-600 deposited on ePTFE membranes. Fibrinogen adsorption decreased with increasing PEG-600 deposited on ePTFE membranes. gamma-Globulin adsorption did not change before or after PEG plasma modification. 1% and 3% PEG-600 plasma-treated ePTFE only slightly increased platelet adhesion, but adhering platelets evidenced no pseudopod formation. 5% PEG-600-modified ePTFE showed relatively large numbers of platelet adhesion. We concluded that 3% PEG-600-modified ePTFE membrane had the best physical properties and biological compatibility, indicating 3% PEG-600-modified ePTFE membranes exhibit the potential for blood filter application. |
| 巻・号 | 60(3) |
| ページ | 502-9 |
| 公開日 | 2002-6-5 |
| DOI | 10.1002/jbm.1294 |
| PII | 10.1002/jbm.1294 |
| PMID | 11920676 |
| MeSH | Adsorption Animals Atmospheric Pressure Biocompatible Materials* Blood Platelets / cytology Cell Adhesion Cell Survival Hemolysis Membranes, Artificial* Microscopy, Electron, Scanning Polyethylene Glycols / chemistry* Polytetrafluoroethylene* / chemistry Rabbits Serum Albumin / chemistry |
| 引用数 | 45 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CHO-K1(RCB0285) |