Reference - Detail
| RRC ID | 41559 |
|---|---|
| Author | Isama K, Tsuchiya T. |
| Title | Effect of gamma-ray irradiated poly(L-lactide) on the differentiation of mouse osteoblast-like MC3T3-E1 cells. |
| Journal | J Biomater Sci Polym Ed |
| Abstract |
The purpose of this study was to clarify the effects of gamma-ray irradiated poly(L-lactide) (PLLA) on the proliferation and differentiation of mouse osteoblast-like MC3T3-E1 cells. The PLLA was y-irradiated at the dose of 10, 25 or 50 kGy, and the molecular weight of irradiated PLLA decreased with increasing irradiation dose. The proliferation and differentiation of MC3T3-E1 cells cultured on irradiated PLLA for 2 weeks were evaluated using micromass culture. The y-irradiation of PLLA did not affect the proliferation, but stimulated the differentiation of MC3T3-E1 cells cultured on irradiated PLLA. These results suggested that lower change in the molecular weight of PLLA was responsible for stimulation of the differentiation of MC3T3-E1 cells cultured on irradiated PLLA. Furthermore, the proliferation and calcification of MC3T3-E1 cells cultured in the medium containing low molecular weight PLLA for 2 weeks were evaluated. The low molecular weight PLLA also stimulated the calcification of MC3T3-E1 cells with no effect on the proliferation. The y-irradiation was suitable for PLLA on the differentiation of mouse osteoblasts. |
| Volume | 13(2) |
| Pages | 153-66 |
| Published | 2002-1-1 |
| DOI | 10.1163/156856202317414348 |
| PMID | 12022747 |
| MeSH | 3T3 Cells Absorbable Implants Animals Biocompatible Materials / chemistry Calcium / metabolism Cell Differentiation Cell Division DNA / metabolism Dose-Response Relationship, Radiation Gamma Rays* Hydroxyproline / chemistry Mice Osteoblasts / metabolism Polyesters / chemistry* Time Factors |
| IF | 2.69 |
| Times Cited | 15 |
| WOS Category | POLYMER SCIENCE ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| Resource | |
| Human and Animal Cells | MC3T3-E1(RCB1126) |