| RRC ID |
41562
|
| 著者 |
Onoue S, Endo K, Yajima T, Kashimoto K.
|
| タイトル |
Pituitary adenylate cyclase activating polypeptide regulates the basal production of nitric oxide in PC12 cells.
|
| ジャーナル |
Life Sci
|
| Abstract |
Vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP), two members of the VIP/secretin/glucagon family, modulate neurotransmission via stimulation of protein kinases including cAMP-dependent protein kinase (PKA) and protein kinase C (PKC) in the central and peripheral nervous systems. They are reported to co-exist with nitric oxide synthases (NOSs) and other neuropeptides within the nervous system and peripheral tissues. In the present study, we investigated the neuronal role of these peptides in NO production in PC12 cells. We showed that PACAP decreased NO production in a dose-dependent manner, and the activators of protein kinase A and C also inhibited the NO production in PC12 cells. RT-PCR experiments demonstrated that PC12 cells constitutively express the mRNAs for neuronal NOS and the PACAP-specific (PAC1) receptor, and we concluded that PACAP plays an important role in the regulation of nNOS activity through PAC1 receptor in PC12 cells.
|
| 巻・号 |
71(2)
|
| ページ |
205-14
|
| 公開日 |
2002-5-31
|
| DOI |
10.1016/s0024-3205(02)01639-9
|
| PII |
S0024320502016399
|
| PMID |
12031689
|
| MeSH |
Analysis of Variance
Animals
Cyclic AMP / metabolism
Mitogens / pharmacology
Neuropeptides / biosynthesis
Neuropeptides / genetics
Neuropeptides / pharmacology*
Nitric Oxide / metabolism*
Nitric Oxide Synthase / genetics
Nitric Oxide Synthase / metabolism
PC12 Cells
Pituitary Adenylate Cyclase-Activating Polypeptide
RNA, Messenger / biosynthesis
Rats
Receptors, Vasoactive Intestinal Peptide / biosynthesis
Receptors, Vasoactive Intestinal Peptide / genetics
|
| IF |
3.647
|
| 引用数 |
17
|
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
PHARMACOLOGY & PHARMACY
|
| リソース情報 |
| ヒト・動物細胞 |
PC-12(RCB0009) |
