論文 - 詳細
| RRC ID | 41565 |
|---|---|
| 著者 | Hayashi M, Tomita M, Yoshizato K. |
| タイトル | Interleukin-2-collagen chimeric protein which liberates interleukin-2 upon collagenolysis. |
| ジャーナル | Protein Eng |
| Abstract |
Interleukin-2 (IL-2) is a potent activator of cellular immunity and has been utilized as an immunotherapeutic agent. We stably immobilized human IL-2 to collagen by covalently binding it to the N-terminus of human type III collagen (3A1) as IL2-3A1 chimeric protein using recombinant technology. The present study was aimed at liberating IL-2 from the immobilized chimeric protein by treating the chimera with bacterial collagenase. These IL2-3A1 chimeras were synthesized in insect cells which had been infected with baculovirus vectors carrying IL2-3A1 cDNA. The IL2-3A1 protein produced was shown to be in a pepsin-resistant triple helical structure and exhibited IL-2 activity to a similar extent as IL-2 itself. IL2-3A1 could be immobilized on the surface of plastic dishes by incubating it in the dishes. The IL-2 region of the immobilized IL2-3A1 was liberated to culture media by collagenase treatment and this freed IL-2 stimulated the growth of lined T cells. Thus, IL2-3A1 chimeric protein could be utilized as an IL-2 deliverer whose T cell mitogenic activity can be liberated by a collagenolytic environment. |
| 巻・号 | 15(5) |
| ページ | 429-36 |
| 公開日 | 2002-5-1 |
| DOI | 10.1093/protein/15.5.429 |
| PMID | 12034863 |
| MeSH | Animals Cell Line Cells, Immobilized / metabolism Chromatography, Affinity Collagen / genetics* Collagen / metabolism Collagenases / metabolism Interleukin-2 / genetics* Interleukin-2 / metabolism Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Spodoptera |
| 引用数 | 8 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | Sf9(RCB0563) |