| RRC ID |
41570
|
| 著者 |
Ichikawa M, Kikuchi T, Tateiwa H, Gotoh N, Ohta K, Arai J, Yoshimura N.
|
| タイトル |
Role of PTB-like protein, a neuronal RNA-binding protein, during the differentiation of PC12 cells.
|
| ジャーナル |
J Biochem
|
| Abstract |
PTB-like protein (PTBLP) is a new homologue of pyrimidine tract binding protein (PTB), and has been cloned as a possible autoantigen in cancer-associated retinopathy. PTBLP has two functional domains, the nuclear localization signal and the RNA recognition motifs (RRMs). Full-length PTBLP (PTBLP-L) has four RRMs, and its alternative splicing product (PTBLP-S) lacks the third and fourth RRMs. Although PTBLPs are expressed in neuronal tissues, the function of PTBLPs has not been determined. We have studed whether PTBLP plays a role in neuronal differentiation using PC12 cells. During the process of nerve growth factor-induced neuronal differentiation of PC12 cells, PTBLP-L was down-regulated whereas PTBLP-S was up-regulated. Transfection of PTBLP-L into PC12 cells led to the suppression of neuronal differentiation. In PTBLP-S transfected cells, however, this suppression was not evident. When both PTBLP-L and PTBLP-S were co-transfected, the suppressive effect of PTBLP-L decreased. In differentiated cells, PTBLP-S localized in the nucleus and PTBLP-L was found dispersed throughout the cytoplasm and neuronal growth cone. These findings suggest that PTBLP-L acts as a negative regulator of neuronal differentiation and PTBLP-S acts as a competitor of PTBLP-L.
|
| 巻・号 |
131(6)
|
| ページ |
861-8
|
| 公開日 |
2002-6-1
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| DOI |
10.1093/oxfordjournals.jbchem.a003176
|
| PMID |
12038983
|
| MeSH |
Animals
Cell Differentiation / physiology*
Nerve Growth Factor / pharmacology
Neurofilament Proteins / biosynthesis
Neurons / cytology*
Neurons / drug effects
Neurons / physiology
PC12 Cells
Polypyrimidine Tract-Binding Protein / genetics
Polypyrimidine Tract-Binding Protein / physiology*
RNA-Binding Proteins / physiology
Rats
Transfection
|
| IF |
2.476
|
| 引用数 |
5
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
