論文 - 詳細
| RRC ID | 41570 |
|---|---|
| 著者 | Ichikawa M, Kikuchi T, Tateiwa H, Gotoh N, Ohta K, Arai J, Yoshimura N. |
| タイトル | Role of PTB-like protein, a neuronal RNA-binding protein, during the differentiation of PC12 cells. |
| ジャーナル | J Biochem |
| Abstract |
PTB-like protein (PTBLP) is a new homologue of pyrimidine tract binding protein (PTB), and has been cloned as a possible autoantigen in cancer-associated retinopathy. PTBLP has two functional domains, the nuclear localization signal and the RNA recognition motifs (RRMs). Full-length PTBLP (PTBLP-L) has four RRMs, and its alternative splicing product (PTBLP-S) lacks the third and fourth RRMs. Although PTBLPs are expressed in neuronal tissues, the function of PTBLPs has not been determined. We have studed whether PTBLP plays a role in neuronal differentiation using PC12 cells. During the process of nerve growth factor-induced neuronal differentiation of PC12 cells, PTBLP-L was down-regulated whereas PTBLP-S was up-regulated. Transfection of PTBLP-L into PC12 cells led to the suppression of neuronal differentiation. In PTBLP-S transfected cells, however, this suppression was not evident. When both PTBLP-L and PTBLP-S were co-transfected, the suppressive effect of PTBLP-L decreased. In differentiated cells, PTBLP-S localized in the nucleus and PTBLP-L was found dispersed throughout the cytoplasm and neuronal growth cone. These findings suggest that PTBLP-L acts as a negative regulator of neuronal differentiation and PTBLP-S acts as a competitor of PTBLP-L. |
| 巻・号 | 131(6) |
| ページ | 861-8 |
| 公開日 | 2002-6-1 |
| DOI | 10.1093/oxfordjournals.jbchem.a003176 |
| PMID | 12038983 |
| MeSH | Animals Cell Differentiation / physiology* Nerve Growth Factor / pharmacology Neurofilament Proteins / biosynthesis Neurons / cytology* Neurons / drug effects Neurons / physiology PC12 Cells Polypyrimidine Tract-Binding Protein / genetics Polypyrimidine Tract-Binding Protein / physiology* RNA-Binding Proteins / physiology Rats Transfection |
| IF | 2.476 |
| 引用数 | 5 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | PC-12(RCB0009) |