Reference - Detail
|Author||Maehara K, Uekawa N, Isobe K.|
|Title||Effects of histone acetylation on transcriptional regulation of manganese superoxide dismutase gene.|
|Journal||Biochem. Biophys. Res. Commun.|
To better understand the link between chromatin modification and manganese superoxide dismutase (Mn-SOD) gene expression, we have investigated the level of histone acetylation at Mn-SOD proximal promoter. TSA induced the expression of Mn-SOD mRNA and its transcriptional activity in C2C12 cells. Sp1 binding sites in the proximal promoter region of Mn-SOD were transcriptionally responsive to TSA by transfection studies. We have detected a localized acetylation of histones H3 and H4, in vivo occupation by Sp1, early growth responsive-1 (Egr-1), and histone deacetylase-1 (HDAC1) in the proximal promoter region of Mn-SOD gene using chromatin immunoprecipitation assays. Our findings indicate that Mn-SOD gene expression is repressed by Sp1-HDAC1 complex. This repression is released by a localized histone acetylation and at least in parts a displacement by Egr-1 in response to TSA.
|MeSH||Acetylation Animals Base Sequence Binding Sites Cell Line DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Enzyme Inhibitors / pharmacology Histone Deacetylase 1 Histone Deacetylases / metabolism Histones / metabolism* Hydroxamic Acids / pharmacology Molecular Sequence Data Promoter Regions, Genetic RNA, Messenger / biosynthesis Response Elements Sp1 Transcription Factor / metabolism Superoxide Dismutase / biosynthesis Superoxide Dismutase / genetics* Transcription Factors / genetics Transcription Factors / metabolism Transcriptional Activation*|
|WOS Category||BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY|
|Human and Animal Cells|