RRC ID 41599
Author Maehara K, Uekawa N, Isobe K.
Title Effects of histone acetylation on transcriptional regulation of manganese superoxide dismutase gene.
Journal Biochem. Biophys. Res. Commun.
Abstract To better understand the link between chromatin modification and manganese superoxide dismutase (Mn-SOD) gene expression, we have investigated the level of histone acetylation at Mn-SOD proximal promoter. TSA induced the expression of Mn-SOD mRNA and its transcriptional activity in C2C12 cells. Sp1 binding sites in the proximal promoter region of Mn-SOD were transcriptionally responsive to TSA by transfection studies. We have detected a localized acetylation of histones H3 and H4, in vivo occupation by Sp1, early growth responsive-1 (Egr-1), and histone deacetylase-1 (HDAC1) in the proximal promoter region of Mn-SOD gene using chromatin immunoprecipitation assays. Our findings indicate that Mn-SOD gene expression is repressed by Sp1-HDAC1 complex. This repression is released by a localized histone acetylation and at least in parts a displacement by Egr-1 in response to TSA.
Volume 295(1)
Pages 187-92
Published 2002-7-5
DOI 10.1016/S0006-291X(02)00646-0
PII S0006-291X(02)00646-0
PMID 12083788
MeSH Acetylation Animals Base Sequence Binding Sites Cell Line DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Enzyme Inhibitors / pharmacology Histone Deacetylase 1 Histone Deacetylases / metabolism Histones / metabolism* Hydroxamic Acids / pharmacology Molecular Sequence Data Promoter Regions, Genetic RNA, Messenger / biosynthesis Response Elements Sp1 Transcription Factor / metabolism Superoxide Dismutase / biosynthesis Superoxide Dismutase / genetics* Transcription Factors / genetics Transcription Factors / metabolism Transcriptional Activation*
IF 2.559
Times Cited 31
Human and Animal Cells