論文 - 詳細
| RRC ID | 41599 |
|---|---|
| 著者 | Maehara K, Uekawa N, Isobe K. |
| タイトル | Effects of histone acetylation on transcriptional regulation of manganese superoxide dismutase gene. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
To better understand the link between chromatin modification and manganese superoxide dismutase (Mn-SOD) gene expression, we have investigated the level of histone acetylation at Mn-SOD proximal promoter. TSA induced the expression of Mn-SOD mRNA and its transcriptional activity in C2C12 cells. Sp1 binding sites in the proximal promoter region of Mn-SOD were transcriptionally responsive to TSA by transfection studies. We have detected a localized acetylation of histones H3 and H4, in vivo occupation by Sp1, early growth responsive-1 (Egr-1), and histone deacetylase-1 (HDAC1) in the proximal promoter region of Mn-SOD gene using chromatin immunoprecipitation assays. Our findings indicate that Mn-SOD gene expression is repressed by Sp1-HDAC1 complex. This repression is released by a localized histone acetylation and at least in parts a displacement by Egr-1 in response to TSA. |
| 巻・号 | 295(1) |
| ページ | 187-92 |
| 公開日 | 2002-7-5 |
| DOI | 10.1016/S0006-291X(02)00646-0 |
| PII | S0006-291X(02)00646-0 |
| PMID | 12083788 |
| MeSH | Acetylation Animals Base Sequence Binding Sites Cell Line DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Enzyme Inhibitors / pharmacology Histone Deacetylase 1 Histone Deacetylases / metabolism Histones / metabolism* Hydroxamic Acids / pharmacology Molecular Sequence Data Promoter Regions, Genetic RNA, Messenger / biosynthesis Response Elements Sp1 Transcription Factor / metabolism Superoxide Dismutase / biosynthesis Superoxide Dismutase / genetics* Transcription Factors / genetics Transcription Factors / metabolism Transcriptional Activation* |
| IF | 2.985 |
| 引用数 | 35 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |