論文 - 詳細
| RRC ID | 41602 |
|---|---|
| 著者 | Shiroki K, Ohsawa C, Sugi N, Wakiyama M, Miura K, Watanabe M, Suzuki Y, Sugano S. |
| タイトル | Internal ribosome entry site-mediated translation of Smad5 in vivo: requirement for a nuclear event. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Smad5 is thought to relay signals of the bone morphogenetic protein pathway. The 5' untranslated region (5'UTR) of human Smad5 mRNA is long, has the potential to form secondary structures and contains five AUG codons. Here we show that the 5'UTR of Smad5 contains an internal ribosome entry site (IRES) located within 100 nt of the 3' end of the 5'UTR. The Smad5 IRES was 4-8-fold more active than the poliovirus IRES in C2C12 cells, which have osteoblastic differentiation ability, but was 5-10-fold less active than the poliovirus IRES in 293T cells. When an in vitro transcript of a dicistronic Smad5 IRES construct was transfected into C2C12 cells, the Smad5 IRES was not able to stimulate the translation of the downstream cistron, although the cap-dependent translation of the upstream cistron was efficient. In contrast, the poliovirus IRES in a dicistronic in vitro transcript was able to stimulate the translation of the downstream cistron to a similar extent as in the case of transfection of the corresponding dicistronic DNA construct. These results suggest that Smad5 IRES activity displays cell specificity and that some as yet unidentified nuclear event may be required for efficient Smad5 IRES-driven translation initiation. |
| 巻・号 | 30(13) |
| ページ | 2851-61 |
| 公開日 | 2002-7-1 |
| DOI | 10.1093/nar/gkf408 |
| PMID | 12087169 |
| PMC | PMC117063 |
| MeSH | 3T3 Cells 5' Untranslated Regions / genetics 5' Untranslated Regions / metabolism Animals Binding Sites / genetics Cell Differentiation Cell Line Cell Nucleus / genetics Cell Nucleus / metabolism DNA-Binding Proteins / genetics* Female HeLa Cells Humans Luciferases / genetics Luciferases / metabolism Mice Oocytes Osteoblasts / cytology Phosphoproteins / genetics* Promoter Regions, Genetic / genetics Protein Biosynthesis RNA, Messenger / genetics RNA, Messenger / metabolism Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Ribosomes / metabolism* Sequence Deletion Smad5 Protein Trans-Activators / genetics* Transcription, Genetic Xenopus |
| IF | 11.502 |
| 引用数 | 25 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) MC3T3-E1(RCB1126) |