論文 - 詳細
| RRC ID | 41603 |
|---|---|
| 著者 | Ono K, Ishihara M, Ishikawa K, Ozeki Y, Deguchi H, Sato M, Hashimoto H, Saito Y, Yura H, Kurita A, Maehara T. |
| タイトル | Periodate-treated, non-anticoagulant heparin-carrying polystyrene (NAC-HCPS) affects angiogenesis and inhibits subcutaneous induced tumour growth and metastasis to the lung. |
| ジャーナル | Br J Cancer |
| Abstract |
Periodate-treated, non-anticoagulant heparin-carrying polystyrene consists of about ten periodate-oxidized, alkaline-degraded low molecular weight-heparin chains linked to a polystyrene core and has a markedly lower anti-coagulant activity than heparin. In this study, we evaluated the effect of non-anticoagulant heparin-carrying polystyrene on tumour growth and metastasis. Non-anticoagulant heparin-carrying polystyrene has a higher activity to inhibit vascular endothelial growth factor-165-, fibroblast growth factor-2- or hepatocyte growth factor-induced human microvascular endothelial cell growth than heparin, ten periodate-oxidized-heparin and ten periodate-oxidized-low molecular weight-heparin, which is probably due to the heparin-clustering effect of non-anticoagulant heparin-carrying polystyrene. Non-anticoagulant heparin-carrying polystyrene inhibited human microvascular endothelial cell, B16 melanoma and Lewis lung cancer cell adhesion to Matrigel-coated plates. Non-anticoagulant heparin-carrying polystyrene also showed strong inhibitory activities in the tubular formation of endothelial cells on Matrigel and B16-melanoma and Lewis lung cancer cell invasion in a Matrigel-coated chamber assay. In vivo studies showed that growth of subcutaneous induced tumours and lung metastasis of B16-melanoma and Lewis lung cancer cells were more effectively inhibited by non-anticoagulant heparin-carrying polystyrene than ten periodate-oxidized-heparin and ten periodate-oxidized-low molecular weight-heparin. Furthermore, non-anticoagulant heparin-carrying polystyrene markedly reduced the number of CD34-positive vessels in subcutaneous Lewis lung cancer tumours, indicating a strong inhibition of angiogenesis. These results suggest that non-anticoagulant heparin-carrying polystyrene has an inhibitory activity on angiogenesis and tumour invasion and may be very useful in cancer therapy. |
| 巻・号 | 86(11) |
| ページ | 1803-12 |
| 公開日 | 2002-6-5 |
| DOI | 10.1038/sj.bjc.6600307 |
| PII | 6600307 |
| PMID | 12087470 |
| PMC | PMC2375397 |
| MeSH | Angiogenesis Inhibitors / pharmacology* Animals Cell Division / drug effects Endothelium, Vascular / cytology* Endothelium, Vascular / drug effects Fibroblasts / cytology Fibroblasts / drug effects Heparin / analogs & derivatives Heparin / pharmacology* Humans Lung Neoplasms / blood supply* Lung Neoplasms / drug therapy Lung Neoplasms / pathology Lung Neoplasms / secondary* Male Mice Mice, Inbred C57BL Microcirculation Neoplasm Invasiveness Neoplasm Metastasis / prevention & control Polystyrenes / pharmacology* Rabbits Swine |
| IF | 5.791 |
| 引用数 | 56 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | B16 melanoma(RCB1283) |