| RRC ID |
41603
|
| 著者 |
Ono K, Ishihara M, Ishikawa K, Ozeki Y, Deguchi H, Sato M, Hashimoto H, Saito Y, Yura H, Kurita A, Maehara T.
|
| タイトル |
Periodate-treated, non-anticoagulant heparin-carrying polystyrene (NAC-HCPS) affects angiogenesis and inhibits subcutaneous induced tumour growth and metastasis to the lung.
|
| ジャーナル |
Br J Cancer
|
| Abstract |
Periodate-treated, non-anticoagulant heparin-carrying polystyrene consists of about ten periodate-oxidized, alkaline-degraded low molecular weight-heparin chains linked to a polystyrene core and has a markedly lower anti-coagulant activity than heparin. In this study, we evaluated the effect of non-anticoagulant heparin-carrying polystyrene on tumour growth and metastasis. Non-anticoagulant heparin-carrying polystyrene has a higher activity to inhibit vascular endothelial growth factor-165-, fibroblast growth factor-2- or hepatocyte growth factor-induced human microvascular endothelial cell growth than heparin, ten periodate-oxidized-heparin and ten periodate-oxidized-low molecular weight-heparin, which is probably due to the heparin-clustering effect of non-anticoagulant heparin-carrying polystyrene. Non-anticoagulant heparin-carrying polystyrene inhibited human microvascular endothelial cell, B16 melanoma and Lewis lung cancer cell adhesion to Matrigel-coated plates. Non-anticoagulant heparin-carrying polystyrene also showed strong inhibitory activities in the tubular formation of endothelial cells on Matrigel and B16-melanoma and Lewis lung cancer cell invasion in a Matrigel-coated chamber assay. In vivo studies showed that growth of subcutaneous induced tumours and lung metastasis of B16-melanoma and Lewis lung cancer cells were more effectively inhibited by non-anticoagulant heparin-carrying polystyrene than ten periodate-oxidized-heparin and ten periodate-oxidized-low molecular weight-heparin. Furthermore, non-anticoagulant heparin-carrying polystyrene markedly reduced the number of CD34-positive vessels in subcutaneous Lewis lung cancer tumours, indicating a strong inhibition of angiogenesis. These results suggest that non-anticoagulant heparin-carrying polystyrene has an inhibitory activity on angiogenesis and tumour invasion and may be very useful in cancer therapy.
|
| 巻・号 |
86(11)
|
| ページ |
1803-12
|
| 公開日 |
2002-6-5
|
| DOI |
10.1038/sj.bjc.6600307
|
| PII |
6600307
|
| PMID |
12087470
|
| PMC |
PMC2375397
|
| MeSH |
Angiogenesis Inhibitors / pharmacology*
Animals
Cell Division / drug effects
Endothelium, Vascular / cytology*
Endothelium, Vascular / drug effects
Fibroblasts / cytology
Fibroblasts / drug effects
Heparin / analogs & derivatives
Heparin / pharmacology*
Humans
Lung Neoplasms / blood supply*
Lung Neoplasms / drug therapy
Lung Neoplasms / pathology
Lung Neoplasms / secondary*
Male
Mice
Mice, Inbred C57BL
Microcirculation
Neoplasm Invasiveness
Neoplasm Metastasis / prevention & control
Polystyrenes / pharmacology*
Rabbits
Swine
|
| IF |
5.791
|
| 引用数 |
56
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
B16 melanoma(RCB1283) |
