論文 - 詳細
| RRC ID | 41604 |
|---|---|
| 著者 | Tsuneoka M, Koda Y, Soejima M, Teye K, Kimura H. |
| タイトル | A novel myc target gene, mina53, that is involved in cell proliferation. |
| ジャーナル | J Biol Chem |
| Abstract |
Myc is a ubiquitous mediator of cell proliferation and can transactivate the expression of various genes through E-box sites. Here we report a novel gene, mina53 (Myc-induced nuclear antigen with a molecular mass of 53 kDa). The mina53 gene encodes a protein with a molecular weight of 53 kDa, which is localized in the nucleus and with part of the protein concentrated in the nucleolus. When serum-starved cells were activated by serum, the level of c-myc mRNA was elevated, and an increase in mina53 mRNA followed the elevation of c-myc mRNA. When expression of c-myc was reduced in human promyelocytic leukemia HL60 cells by phorbol 12-myristate 13-acetate, the expression of mina53 mRNA and protein was reduced. The expression of mina53 mRNA and Mina53 protein was induced by ectopic introduction of wild type c-Myc but not by a mutant c-Myc lacking the transactivation domain. When c-Myc in the c-MycER chimeric protein was activated, mina53 mRNA was increased, even in the presence of an inhibitor for protein synthesis. E-box sites are present in a region proximal to the transcription initiation sites of the mina53 gene. The gene expression from the mina53 promoter was elevated by c-Myc through E-box sites. c-Myc protein bound to the mina53 promoter region in vivo in HL60 cells in the proliferating phase but not after treatment of cells with phorbol 12-myristate 13-acetate. Specific inhibition of mina53 expression by an RNA interference method severely suppressed cell proliferation. Taken together, these results indicate that mina53 is a direct target gene of Myc, suggesting that mina53 is involved in mammalian cell proliferation. |
| 巻・号 | 277(38) |
| ページ | 35450-9 |
| 公開日 | 2002-9-20 |
| DOI | 10.1074/jbc.M204458200 |
| PII | S0021-9258(18)36782-6 |
| PMID | 12091391 |
| MeSH | Amino Acid Sequence Animals Base Sequence Cell Division / genetics* DNA Primers Dioxygenases Gene Expression Regulation / drug effects Genes, myc* Histone Demethylases Humans Molecular Sequence Data Nuclear Proteins / chemistry Nuclear Proteins / genetics* Promoter Regions, Genetic RNA, Messenger / genetics Sequence Homology, Amino Acid Tetradecanoylphorbol Acetate / pharmacology Tumor Cells, Cultured |
| IF | 4.238 |
| 引用数 | 84 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RCN-9(RCB0511) |