| RRC ID |
41604
|
| 著者 |
Tsuneoka M, Koda Y, Soejima M, Teye K, Kimura H.
|
| タイトル |
A novel myc target gene, mina53, that is involved in cell proliferation.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
Myc is a ubiquitous mediator of cell proliferation and can transactivate the expression of various genes through E-box sites. Here we report a novel gene, mina53 (Myc-induced nuclear antigen with a molecular mass of 53 kDa). The mina53 gene encodes a protein with a molecular weight of 53 kDa, which is localized in the nucleus and with part of the protein concentrated in the nucleolus. When serum-starved cells were activated by serum, the level of c-myc mRNA was elevated, and an increase in mina53 mRNA followed the elevation of c-myc mRNA. When expression of c-myc was reduced in human promyelocytic leukemia HL60 cells by phorbol 12-myristate 13-acetate, the expression of mina53 mRNA and protein was reduced. The expression of mina53 mRNA and Mina53 protein was induced by ectopic introduction of wild type c-Myc but not by a mutant c-Myc lacking the transactivation domain. When c-Myc in the c-MycER chimeric protein was activated, mina53 mRNA was increased, even in the presence of an inhibitor for protein synthesis. E-box sites are present in a region proximal to the transcription initiation sites of the mina53 gene. The gene expression from the mina53 promoter was elevated by c-Myc through E-box sites. c-Myc protein bound to the mina53 promoter region in vivo in HL60 cells in the proliferating phase but not after treatment of cells with phorbol 12-myristate 13-acetate. Specific inhibition of mina53 expression by an RNA interference method severely suppressed cell proliferation. Taken together, these results indicate that mina53 is a direct target gene of Myc, suggesting that mina53 is involved in mammalian cell proliferation.
|
| 巻・号 |
277(38)
|
| ページ |
35450-9
|
| 公開日 |
2002-9-20
|
| DOI |
10.1074/jbc.M204458200
|
| PII |
S0021-9258(18)36782-6
|
| PMID |
12091391
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Cell Division / genetics*
DNA Primers
Dioxygenases
Gene Expression Regulation / drug effects
Genes, myc*
Histone Demethylases
Humans
Molecular Sequence Data
Nuclear Proteins / chemistry
Nuclear Proteins / genetics*
Promoter Regions, Genetic
RNA, Messenger / genetics
Sequence Homology, Amino Acid
Tetradecanoylphorbol Acetate / pharmacology
Tumor Cells, Cultured
|
| IF |
4.238
|
| 引用数 |
84
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RCN-9(RCB0511) |
