| RRC ID |
41623
|
| Author |
Long Z, Sekine M, Adachi M, Furuchi T, Imai K, Nimura N, Homma H.
|
| Title |
Cell density inversely regulates D- and L-aspartate levels in rat pheochromocytoma MPT1 cells.
|
| Journal |
Arch Biochem Biophys
|
| Abstract |
In a previous report (FEBS Lett. 434 (1998) 231), we demonstrated for the first time that D-aspartate (D-Asp) is synthesized in rat pheochromocytoma 12 (PC12) cells. This unique amino acid is believed to act as a novel messenger in mammalian cell regulation. However, the dynamics of D-Asp homeostasis in mammalian cells is yet to be elucidated. In this communication, we demonstrate that D-Asp is also synthesized in MPT1 cells (a subclone of PC12 cells) and that the D- and L-Asp levels in cells are regulated by cell density of the culture. Our data show that D-Asp levels increase, while in contrast, L-Asp levels decrease as a function of increased cell density. Conversely, in PC12 cells, which do not express the glutamate transporter involved in the incorporation of D- and L-Asp into cells, L-Asp levels decrease upon cell density increase while D-Asp concentrations remain almost unchanged. The results indicate that the biochemical behaviors of D- and L-Asp in mammalian cells are distinct and that the cellular levels of these stereoisomers appear to be under different control mechanisms.
|
| Volume |
404(1)
|
| Pages |
92-7
|
| Published |
2002-8-1
|
| DOI |
10.1016/s0003-9861(02)00241-2
|
| PII |
S0003986102002412
|
| PMID |
12127073
|
| MeSH |
Animals
Aspartic Acid / biosynthesis
Aspartic Acid / chemistry
Aspartic Acid / metabolism*
Cell Count
Homeostasis
PC12 Cells
Pheochromocytoma / metabolism*
Pheochromocytoma / pathology
Rats
Stereoisomerism
|
| IF |
3.391
|
| Times Cited |
20
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
PC-12(RCB0009) |
