| RRC ID |
41627
|
| 著者 |
Sun HB, Malacinski GM, Yokota H.
|
| タイトル |
Promoter competition assay for analyzing gene regulation in joint tissue engineering.
|
| ジャーナル |
Front Biosci
|
| Abstract |
We describe a new biochemical technique, "promoter competition assay," for examining the role of cis-acting DNA elements in tissue cultures. Recent advances in tissue engineering permit the culture of a variety of cells. Many tissues are engineered, however, without an appropriate understanding of molecular machinery that regulates gene expression and cellular growth. For elucidating the role of cis-acting regulatory elements in cellular differentiation and growth, we developed the promoter competition assay. This assay uses a transient transfer into cells of double-stranded DNA fragments consisting of cis-acting regulatory elements. The transferred DNA fragments act as a competitor and titrate the function of their genomic counterparts. Using synovial cells derived from a rheumatoid arthritis patient, we examined a role of NF-kappa B binding sites in the regulation of the expression of matrix metalloproteinase (MMP) genes. The results support a stimulatory role of NF-kappa B in transcriptional regulation of MMP-1 and MMP-13.
|
| 巻・号 |
7
|
| ページ |
a169-74
|
| 公開日 |
2002-8-1
|
| DOI |
10.2741/a751
|
| PMID |
12133815
|
| MeSH |
Arthritis, Rheumatoid / metabolism
Arthritis, Rheumatoid / pathology
Binding, Competitive / genetics
Cell Line
Cell Line, Transformed
DNA / genetics
DNA / physiology
Gene Expression Regulation / genetics*
Humans
Knee / pathology
Matrix Metalloproteinases / biosynthesis
NF-kappa B / genetics
Promoter Regions, Genetic / genetics*
Protein Binding / genetics
RNA, Messenger / biosynthesis
RNA, Messenger / physiology
Regulatory Sequences, Nucleic Acid / genetics
Stress, Mechanical
Synovial Membrane / metabolism*
Tissue Engineering / methods*
|
| IF |
2.747
|
| 引用数 |
18
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MH7A(RCB1512) |
