論文 - 詳細
| RRC ID | 41627 |
|---|---|
| 著者 | Sun HB, Malacinski GM, Yokota H. |
| タイトル | Promoter competition assay for analyzing gene regulation in joint tissue engineering. |
| ジャーナル | Front Biosci |
| Abstract |
We describe a new biochemical technique, "promoter competition assay," for examining the role of cis-acting DNA elements in tissue cultures. Recent advances in tissue engineering permit the culture of a variety of cells. Many tissues are engineered, however, without an appropriate understanding of molecular machinery that regulates gene expression and cellular growth. For elucidating the role of cis-acting regulatory elements in cellular differentiation and growth, we developed the promoter competition assay. This assay uses a transient transfer into cells of double-stranded DNA fragments consisting of cis-acting regulatory elements. The transferred DNA fragments act as a competitor and titrate the function of their genomic counterparts. Using synovial cells derived from a rheumatoid arthritis patient, we examined a role of NF-kappa B binding sites in the regulation of the expression of matrix metalloproteinase (MMP) genes. The results support a stimulatory role of NF-kappa B in transcriptional regulation of MMP-1 and MMP-13. |
| 巻・号 | 7 |
| ページ | a169-74 |
| 公開日 | 2002-8-1 |
| DOI | 10.2741/a751 |
| PMID | 12133815 |
| MeSH | Arthritis, Rheumatoid / metabolism Arthritis, Rheumatoid / pathology Binding, Competitive / genetics Cell Line Cell Line, Transformed DNA / genetics DNA / physiology Gene Expression Regulation / genetics* Humans Knee / pathology Matrix Metalloproteinases / biosynthesis NF-kappa B / genetics Promoter Regions, Genetic / genetics* Protein Binding / genetics RNA, Messenger / biosynthesis RNA, Messenger / physiology Regulatory Sequences, Nucleic Acid / genetics Stress, Mechanical Synovial Membrane / metabolism* Tissue Engineering / methods* |
| IF | 2.747 |
| 引用数 | 18 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | MH7A(RCB1512) |