Reference - Detail
| RRC ID | 41628 |
|---|---|
| Author | Kato J, Kato N, Moriyama M, Goto T, Taniguchi H, Shiratori Y, Omata M. |
| Title | Interferons specifically suppress the translation from the internal ribosome entry site of hepatitis C virus through a double-stranded RNA-activated protein kinase-independent pathway. |
| Journal | J Infect Dis |
| Abstract |
Interferon (IFN) therapy is used worldwide as the best available treatment for hepatitis C virus (HCV) infection; however, little is known about how IFN or other drugs work against liver diseases. The effect of 6 drugs (glycyrrhizin, ursodeoxycholic acid, ribavirin, methylprednisolone, IFN-alpha, and IFN-beta) on HCV RNA translation from the HCV internal ribosome entry site (IRES) was investigated, using a bicistronic reporter containing the HCV IRES. IFNs suppressed both cap-dependent and HCV IRES-dependent translation, with HCV IRES-dependent translation being more significantly suppressed. In contrast to HCV IRES, IFN did not suppress either foot-and-mouth disease virus IRES-dependent or encephalomyocarditis virus IRES-dependent translation more than it suppressed cap-dependent translation. Moreover, dominant inhibition of HCV IRES-dependent over cap-dependent translation depended neither on the double-stranded RNA-activated protein kinase activation nor on La protein function. These results indicate a novel antiviral effect of IFNs against HCV. |
| Volume | 186(2) |
| Pages | 155-63 |
| Published | 2002-7-15 |
| DOI | 10.1086/341467 |
| PII | JID011268 |
| PMID | 12134250 |
| MeSH | Anti-Infective Agents / pharmacology* Antiviral Agents / pharmacology Autoantigens / biosynthesis Blotting, Western Cholagogues and Choleretics / pharmacology Encephalomyocarditis virus / metabolism Foot-and-Mouth Disease Virus / metabolism Glucocorticoids / pharmacology Glycyrrhizic Acid / pharmacology Hepacivirus / drug effects* Hepacivirus / genetics Hepacivirus / metabolism Humans Interferons / metabolism Interferons / pharmacology* Methylprednisolone / pharmacology Protein Biosynthesis / drug effects Protein Biosynthesis / physiology RNA, Double-Stranded / genetics RNA, Double-Stranded / metabolism RNA, Viral / genetics RNA, Viral / metabolism Ribavirin / pharmacology Ribonucleoproteins / biosynthesis Ribosomes / drug effects* Ribosomes / genetics Ribosomes / metabolism Tumor Cells, Cultured Ursodeoxycholic Acid / pharmacology |
| IF | 5.022 |
| Times Cited | 23 |
| WOS Category | INFECTIOUS DISEASES IMMUNOLOGY MICROBIOLOGY |
| Resource | |
| Human and Animal Cells | HuH-7(RCB1366) |