RRC ID 41645
Author Nakahara K, Trakoontivakorn G, Alzoreky NS, Ono H, Onishi-Kameyama M, Yoshida M.
Title Antimutagenicity of some edible Thai plants, and a bioactive carbazole alkaloid, mahanine, isolated from Micromelum minutum.
Journal J. Agric. Food Chem.
Abstract The antimutagenic activity against Trp-P-1 of methanolic extracts of 118 samples (108 species) of edible Thai plants was examined by the Ames Test. The activity was evaluated by the amount of plant extracts which suppressed 90% of the mutagenesis (ED90). Five plants, Micromelum minutum, Oroxylum indicum, Cuscuta chinensis, Azadirachta indica, and Litsea petiolata, exhibited significant activity with antimutagenic ED90 values lower than 5 microL/plate (0.1 mg of dry plant material equivalent). The activity-guided fractionation of the extract of M. minutum, which exhibited the highest antimutagenic activity in the screening, resulted in the isolation of an active principle, (+)-mahanine (1) as confirmed by its physicochemical properties. Compound 1 showed a wide variety of biological activity, including antimutagenicity against heterocyclic amines such as Trp-P-1 with an IC50 of 5.2 microM, cytotoxicity against a tumor cell line HL60 with a MIC100 of 4.0 microg/mL, and antimicrobial activity against Bacillus cereus and Staphylococcus aureus with MIC100 values of 6.25 and 12.5 microg/mL, respectively.
Volume 50(17)
Pages 4796-802
Published 2002-8-14
DOI 10.1021/jf025564w
PII jf025564w
PMID 12166962
MeSH Anti-Bacterial Agents / pharmacology Antimutagenic Agents / pharmacology* Antineoplastic Agents / pharmacology Bacillus cereus / drug effects Bacillus cereus / growth & development Bignoniaceae / chemistry Carbazoles / isolation & purification Carbazoles / pharmacology* Chemical Phenomena Chemistry, Physical Convolvulaceae / chemistry Lauraceae / chemistry Meliaceae / chemistry Mutagenicity Tests Plant Extracts / pharmacology* Rutaceae / chemistry* Staphylococcus aureus / drug effects Staphylococcus aureus / growth & development Thailand Tumor Cells, Cultured
IF 3.571
Times Cited 82
Human and Animal Cells