Reference - Detail
|Author||Nakahara K, Trakoontivakorn G, Alzoreky NS, Ono H, Onishi-Kameyama M, Yoshida M.|
|Title||Antimutagenicity of some edible Thai plants, and a bioactive carbazole alkaloid, mahanine, isolated from Micromelum minutum.|
|Journal||J. Agric. Food Chem.|
The antimutagenic activity against Trp-P-1 of methanolic extracts of 118 samples (108 species) of edible Thai plants was examined by the Ames Test. The activity was evaluated by the amount of plant extracts which suppressed 90% of the mutagenesis (ED90). Five plants, Micromelum minutum, Oroxylum indicum, Cuscuta chinensis, Azadirachta indica, and Litsea petiolata, exhibited significant activity with antimutagenic ED90 values lower than 5 microL/plate (0.1 mg of dry plant material equivalent). The activity-guided fractionation of the extract of M. minutum, which exhibited the highest antimutagenic activity in the screening, resulted in the isolation of an active principle, (+)-mahanine (1) as confirmed by its physicochemical properties. Compound 1 showed a wide variety of biological activity, including antimutagenicity against heterocyclic amines such as Trp-P-1 with an IC50 of 5.2 microM, cytotoxicity against a tumor cell line HL60 with a MIC100 of 4.0 microg/mL, and antimicrobial activity against Bacillus cereus and Staphylococcus aureus with MIC100 values of 6.25 and 12.5 microg/mL, respectively.
|MeSH||Anti-Bacterial Agents / pharmacology Antimutagenic Agents / pharmacology* Antineoplastic Agents / pharmacology Bacillus cereus / drug effects Bacillus cereus / growth & development Bignoniaceae / chemistry Carbazoles / isolation & purification Carbazoles / pharmacology* Chemical Phenomena Chemistry, Physical Convolvulaceae / chemistry Lauraceae / chemistry Meliaceae / chemistry Mutagenicity Tests Plant Extracts / pharmacology* Rutaceae / chemistry* Staphylococcus aureus / drug effects Staphylococcus aureus / growth & development Thailand Tumor Cells, Cultured|
|WOS Category||FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED AGRICULTURE, MULTIDISCIPLINARY|
|Human and Animal Cells|