RRC ID 41664
Author Kikuchi T, Akasaki Y, Abe T, Ohno T.
Title Intratumoral injection of dendritic and irradiated glioma cells induces anti-tumor effects in a mouse brain tumor model.
Journal Cancer Immunol. Immunother.
Abstract Malignant astrocytoma is the most common primary brain tumor in adults. The median survival of patients with malignant astrocytomas (high-grade astrocytomas) is about 1-2 years, despite aggressive treatment that includes surgical resection, radiotherapy and cytotoxic chemotherapy. Therefore, novel therapeutic approaches are needed to prolong survival. We investigated antitumor immunity conferred by the intratumoral injection of dendritic (DC) and irradiated glioma cells (IR-GC) in a mouse brain tumor model. Intratumorally injected DC migrated to the lymph nodes and elicited systemic immunity against autologous glioma cells. In a treatment model, intratumoral injection of DC and IR-GC prolonged the survival of brain tumor-bearing mice. Efficacy was reduced when studies were performed in mice depleted of CD8(+) T cells. Administration of DC or IR-GC alone had no effect on survival of brain tumor-bearing mice. CTL activity against glioma cells from immunized mice was also stimulated by coadministration of DC and IR-GC compared with the controls. These results support the therapeutic efficacy of intratumoral injection of DC and IR-GC.
Volume 51(8)
Pages 424-30
Published 2002-10
DOI 10.1007/s00262-002-0297-z
PMID 12202903
MeSH Animals Astrocytoma / therapy Brain Neoplasms / therapy* CD8-Positive T-Lymphocytes / metabolism Cell Movement Dendrites / immunology* Dendrites / metabolism Glioma / immunology* Glioma / metabolism Immunotherapy / methods* Killer Cells, Natural / cytology Lymph Nodes / metabolism Mice Time Factors Tumor Cells, Cultured
IF 4.225
Times Cited 20
WOS Category IMMUNOLOGY ONCOLOGY
Resource
Human and Animal Cells