| RRC ID |
41672
|
| Author |
Tsutsumimoto T, Wakabayashi S, Kinoshita T, Horiuchi H, Takaoka K.
|
| Title |
A phosphodiesterase inhibitor, pentoxifylline, enhances the bone morphogenetic protein-4 (BMP-4)-dependent differentiation of osteoprogenitor cells.
|
| Journal |
Bone
|
| Abstract |
Bone morphogenetic protein-4 (BMP-4), a member of the transforming growth factor-beta superfamily, is capable of initiating differentiation of uncommitted mesenchymal cells into a chondro/osteogenic pathway. This study reports the effects of pentoxifylline (PTX), a nonspecific inhibitor of phosphodiesterases (PDEs), that causes elevation of the intracellular cyclic adenosine monophosphate (cAMP) level on the BMP-4-induced chondro/osteogenic differentiation of a mesenchymal cell line, C3H10T1/2; a bone marrow stromal cell line, ST2; and an osteoblastic cell line, MC3T3-E1. It was found that PTX enhanced BMP-4-induced chondro/osteogenic differentiation in C3H10T1/2 and ST2 cells. Similar effects were observed when adding dibutyryl-cAMP and forskolin. These results indicate that cAMP may potentiate the action of BMP-4 on osteoprogenitor cells, highlighting the possibility that PDE inhibitors could be used as therapeutic agents to enhance bone formation through this effect.
|
| Volume |
31(3)
|
| Pages |
396-401
|
| Published |
2002-9-1
|
| DOI |
10.1016/s8756-3282(02)00839-6
|
| PII |
S8756328202008396
|
| PMID |
12231412
|
| MeSH |
Animals
Bone Morphogenetic Protein 4
Bone Morphogenetic Proteins / metabolism
Bone Morphogenetic Proteins / physiology*
CHO Cells
Cell Differentiation / drug effects*
Cell Differentiation / physiology
Cell Line
Cricetinae
Cyclic AMP / metabolism
Drug Synergism
Mice
Mice, Inbred C3H
Osteoblasts / cytology
Osteoblasts / drug effects*
Osteoblasts / metabolism
Pentoxifylline / pharmacology*
Phosphodiesterase Inhibitors / pharmacology*
Stem Cells / cytology
Stem Cells / drug effects*
Stem Cells / metabolism
|
| IF |
4.147
|
| Times Cited |
31
|
|
WOS Category
|
ENDOCRINOLOGY & METABOLISM
|
| Resource |
| Human and Animal Cells |
MC3T3-E1(RCB1126)
ST2(RCB0224) |
