論文 - 詳細
| RRC ID | 41677 |
|---|---|
| 著者 | Yamanaka R, Zullo SA, Ramsey J, Yajima N, Tsuchiya N, Tanaka R, Blaese M, Xanthopoulos KG. |
| タイトル | Marked enhancement of antitumor immune responses in mouse brain tumor models by genetically modified dendritic cells producing Semliki Forest virus-mediated interleukin-12. |
| ジャーナル | J Neurosurg |
| Abstract |
OBJECT:The authors evaluated dendritic cell (DC)-based immunotherapy for malignant brain tumor to improve its therapeutic efficacy. METHODS:Dendritic cells were isolated from bone marrow and pulsed with phosphate-buffered saline, Semliki Forest virus (SFV)-LacZ, retrovirus vector GCsap-interleukin (IL)-12, and SFV-IL-12, respectively, to treat mice bearing brain tumors of the B16 cell line. The results indicated that therapeutic immunization with DCs pulsed with SFV-IL-12 prolonged the survival of mice with established tumors. Semliki Forest virus induced apoptosis in DCs, which in turn facilitated the uptake of apoptotic cells by other DCs, thus providing a potential mechanism for enhanced immunogenicity. CONCLUSIONS:Therapy with DCs that have been pulsed with SFV-mediated IL-12 may be an excellent step in the development of new cancer vaccines. Intratumorally injected DCs that have been transiently transduced with IL-12 do not require pulsing of a source of tumor antigens to induce tumor regression. |
| 巻・号 | 97(3) |
| ページ | 611-8 |
| 公開日 | 2002-9-1 |
| DOI | 10.3171/jns.2002.97.3.0611 |
| PMID | 12296646 |
| MeSH | Animals Apoptosis / immunology Bone Marrow Cells Brain Neoplasms / immunology Brain Neoplasms / therapy* CD4-Positive T-Lymphocytes / immunology CD4-Positive T-Lymphocytes / metabolism CD8-Positive T-Lymphocytes / immunology Dendritic Cells / immunology* Dendritic Cells / virology Genetic Therapy Genetic Vectors Glioma / immunology Glioma / therapy* Immunotherapy, Active Interferon-gamma / biosynthesis Interleukin-12 / genetics* Interleukin-12 / immunology* Mice Mice, Inbred C57BL Semliki forest virus / genetics* Transduction, Genetic Tumor Cells, Cultured / transplantation |
| IF | 3.968 |
| 引用数 | 42 |
| WOS 分野 | SURGERY CLINICAL NEUROLOGY |
| リソース情報 | |
| ヒト・動物細胞 | B16 |