論文 - 詳細
| RRC ID | 41679 |
|---|---|
| 著者 | Suyama E, Kawasaki H, Taira K. |
| タイトル | Identification of a caspase 3-independent role of pro-apoptotic factor Bak in TNF-alpha-induced apoptosis. |
| ジャーナル | FEBS Lett |
| Abstract |
By using our recently developed gene discovery system, we have identified Bak, a member of the Bcl-2 family, as a pro-apoptotic factor in the tumor necrosis factor (TNF)-alpha-induced apoptotic pathway in caspase 3-deficient cells. Unlike Bcl-2, Bak stimulates several apoptotic pathways, however the molecular mechanism(s) of its action remains unclear. For example, it is unclear whether Bak induces apoptosis in caspase 3-deficient cells. In this study, we examined the effects of overexpression of Bak in MCF-7 cells that lack caspase 3. We found that despite the absence of caspase 3 in MCF-7 cells, they were more sensitive to the cell death effects of Bak as compared to caspase 3-expressing HeLa S3 cells. The targeting of Bak function by ribozymes suggests that Bak is required for the TNF-alpha-induced apoptotic pathway in caspase 3-deficient cells. This study demonstrates the caspase 3-independent function of Bak in the TNF-alpha-induced apoptotic pathway. |
| 巻・号 | 528(1-3) |
| ページ | 63-9 |
| 公開日 | 2002-9-25 |
| DOI | 10.1016/s0014-5793(02)03193-9 |
| PII | S0014579302031939 |
| PMID | 12297281 |
| MeSH | Apoptosis / drug effects* Apoptosis / physiology* Base Sequence Caspase 3 Caspases / deficiency Caspases / physiology* Cell Line Gene Expression Green Fluorescent Proteins HeLa Cells Humans Luminescent Proteins / genetics Luminescent Proteins / metabolism Membrane Proteins / genetics Membrane Proteins / physiology* Molecular Sequence Data Nucleic Acid Conformation RNA, Catalytic / chemistry RNA, Catalytic / genetics RNA, Catalytic / metabolism RNA, Messenger / chemistry RNA, Messenger / genetics RNA, Messenger / metabolism Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / physiology Transfection Tumor Necrosis Factor-alpha / pharmacology* bcl-2 Homologous Antagonist-Killer Protein |
| IF | 3.057 |
| 引用数 | 17 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |