論文 - 詳細
| RRC ID | 41700 |
|---|---|
| 著者 | Yuasa T, Kataoka H, Kinto N, Iwamoto M, Enomoto-Iwamoto M, Iemura S, Ueno N, Shibata Y, Kurosawa H, Yamaguchi A. |
| タイトル | Sonic hedgehog is involved in osteoblast differentiation by cooperating with BMP-2. |
| ジャーナル | J Cell Physiol |
| Abstract |
The roles of Sonic hedgehog (Shh) and Bone morphogenetic protein-2 (Bmp-2) in osteoblast differentiation were investigated using in vitro cell systems. Recombinant amino-terminal portion of SHH (rSHH-N) dose dependently stimulated ALP activity in C3H10T1/2 and MC3T3-E1 cells. rSHH-N induced expression of Osteocalcin mRNA in C3H10T1/2 cells. A soluble form of the receptor for type IA BMP receptor antagonized rSHH-N-induced ALP activity in C3H10T1/2 and MC3T3-E1 cells, indicating that BMPs are involved in SHH-induced osteoblast differentiation. Simultaneous supplement with rSHH-N and BMP-2 synergistically induced ALP activity and expression of Osteocalcin mRNA in C3H10T1/2 cells. Pretreatment with rSHH-N for 6 h enhanced the response to BMP-2 by increasing ALP activity in C3H10T1/2 and MC3T3-E1 cells. Stimulatory effects of rSHH-N and additive effects with rSHH-N and BMP-2 on ALP activity were also observed in mouse primary osteoblastic cells. Transplantation of BMP-2 (1 microg) into muscle of mice induced formation of ectopic bone, whereas transplantation of r-SHH-N (1-5 microg) failed to generate it. These results indicate that Shh plays important roles in osteoblast differentiation by cooperating with BMP. |
| 巻・号 | 193(2) |
| ページ | 225-32 |
| 公開日 | 2002-11-1 |
| DOI | 10.1002/jcp.10166 |
| PMID | 12385000 |
| MeSH | Alkaline Phosphatase / analysis Animals Bone Development Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins / physiology* Cell Differentiation Cell Line Collagen Type I / metabolism Dose-Response Relationship, Drug Embryonic Induction Hedgehog Proteins Humans Mice Mice, Inbred C3H Osteoblasts / cytology* Osteoblasts / drug effects Osteoblasts / enzymology Osteoblasts / transplantation Osteocalcin / drug effects Osteocalcin / metabolism Peptide Fragments / metabolism RNA, Messenger / metabolism Recombinant Proteins / pharmacology Time Factors Trans-Activators / physiology* Transforming Growth Factor beta / metabolism Transplants |
| IF | 5.546 |
| 引用数 | 74 |
| WOS 分野 | PHYSIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | 10T1/2(RCB0247) |