論文 - 詳細
| RRC ID | 41701 |
|---|---|
| 著者 | Nakamura E, Hagen SJ. |
| タイトル | Role of glutamine and arginase in protection against ammonia-induced cell death in gastric epithelial cells. |
| ジャーナル | Am J Physiol Gastrointest Liver Physiol |
| Abstract |
Ammonia is a cytotoxic factor produced during Helicobacter pylori infection that may reduce the survival of surface epithelial cells. Here we examine whether ammonia kills cells and whether L-glutamine (L-Gln) protects against cell death by stimulating ammonia detoxification pathways. Cell viability and vacuolation were quantified in rat gastric epithelial (RGM1) cells incubated with ammonium chloride at pH 7.4 in the presence or absence of L-Gln. Incubation of RGM1 cells with ammonium chloride caused a dose-dependent increase in cell death and vacuolation, which were both inhibited by L-Gln. We show that RGM1 cells metabolize ammonia to urea via arginase, a process that is stimulated by L-Gln and results in reduced ammonia cytotoxicity. L-Gln also inhibits the uptake and facilitates the extrusion of ammonia from cells. Blockade of glutamine synthetase did not reduce the survival of RGM1 cells, demonstrating that the conversion of L-glutamate and ammonia to L-Gln is not involved in ammonia detoxification. Thus our data support a role for L-Gln and arginase in protection against ammonia-induced cell death in gastric epithelial cells. |
| 巻・号 | 283(6) |
| ページ | G1264-75 |
| 公開日 | 2002-12-1 |
| DOI | 10.1152/ajpgi.00235.2002 |
| PII | 00235.2002 |
| PMID | 12388179 |
| MeSH | Ammonia / metabolism Ammonium Chloride / pharmacology* Animals Arginase / physiology* Cell Death / drug effects* Cell Line Epithelial Cells / ultrastructure Glutamic Acid / metabolism Glutamine / biosynthesis Glutamine / pharmacology Glutamine / physiology* Helicobacter Infections Helicobacter pylori / metabolism Kinetics Methylamines / pharmacology Rats Stomach / ultrastructure* Urea / metabolism Vacuoles / drug effects |
| IF | 3.725 |
| 引用数 | 28 |
| WOS 分野 | GASTROENTEROLOGY & HEPATOLOGY PHYSIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RGM1(RCB0876) |