| RRC ID |
41715
|
| 著者 |
Okazaki M, Yamada Y, Nishimoto N, Yoshizaki K, Mihara M.
|
| タイトル |
Characterization of anti-mouse interleukin-6 receptor antibody.
|
| ジャーナル |
Immunol Lett
|
| Abstract |
Hybridoma that produces rat anti-mouse interleukin 6 receptor (IL-6R) antibody, MR16-1, was established by the fusion of mouse P3U1 myeloma cells and spleen cells from mouse soluble IL-6R (sIL-6R)-immunized Wistar rat. In the present study, we examined the characteristics of MR16-1 in vitro and in vivo. MR16-1 bound to mouse sIL-6R dose-dependently. MR16-1 suppressed IL-6-induced proliferation of 7TD1 cells in a dose-dependent manner and this inhibitory effect was reversed by the addition of a higher concentration of IL-6. Cross-reactivity study using T cells from mouse, rat, and human revealed that MR16-1 did not cross-react with human and rat IL-6R. Binding region analysis using several human-mouse chimeric IL-6Rs showed that half of the fibronectin domain II of mouse IL-6R (amino acids 214-285) was required for MR16-1 binding. Furthermore, MR16-1 completely suppressed IL-6-induced antibody production in DNP-KLH immunized mice. These lines of evidence demonstrate that MR16-1 is useful to investigate the physiological and pathological roles of IL-6 and sIL-6R in mice.
|
| 巻・号 |
84(3)
|
| ページ |
231-40
|
| 公開日 |
2002-12-3
|
| DOI |
10.1016/s0165-2478(02)00202-x
|
| PII |
S016524780200202X
|
| PMID |
12413742
|
| MeSH |
Amino Acid Sequence
Animals
Antibodies, Monoclonal / chemistry*
Antibodies, Monoclonal / immunology*
Antibodies, Monoclonal / pharmacology
Antibody Formation / drug effects
Antibody Formation / immunology
Antibody Specificity / immunology*
COS Cells
Humans
Hybridomas / drug effects
Hybridomas / immunology
Interleukin-6 / immunology
Interleukin-6 / pharmacology
Mice
Molecular Sequence Data
Rats
Receptors, Interleukin-6 / antagonists & inhibitors
Receptors, Interleukin-6 / immunology*
Species Specificity
|
| IF |
3.276
|
| 引用数 |
74
|
|
WOS 分野
|
IMMUNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
COS-7(RCB0539) |
