論文 - 詳細
| RRC ID | 41715 |
|---|---|
| 著者 | Okazaki M, Yamada Y, Nishimoto N, Yoshizaki K, Mihara M. |
| タイトル | Characterization of anti-mouse interleukin-6 receptor antibody. |
| ジャーナル | Immunol Lett |
| Abstract |
Hybridoma that produces rat anti-mouse interleukin 6 receptor (IL-6R) antibody, MR16-1, was established by the fusion of mouse P3U1 myeloma cells and spleen cells from mouse soluble IL-6R (sIL-6R)-immunized Wistar rat. In the present study, we examined the characteristics of MR16-1 in vitro and in vivo. MR16-1 bound to mouse sIL-6R dose-dependently. MR16-1 suppressed IL-6-induced proliferation of 7TD1 cells in a dose-dependent manner and this inhibitory effect was reversed by the addition of a higher concentration of IL-6. Cross-reactivity study using T cells from mouse, rat, and human revealed that MR16-1 did not cross-react with human and rat IL-6R. Binding region analysis using several human-mouse chimeric IL-6Rs showed that half of the fibronectin domain II of mouse IL-6R (amino acids 214-285) was required for MR16-1 binding. Furthermore, MR16-1 completely suppressed IL-6-induced antibody production in DNP-KLH immunized mice. These lines of evidence demonstrate that MR16-1 is useful to investigate the physiological and pathological roles of IL-6 and sIL-6R in mice. |
| 巻・号 | 84(3) |
| ページ | 231-40 |
| 公開日 | 2002-12-3 |
| DOI | 10.1016/s0165-2478(02)00202-x |
| PII | S016524780200202X |
| PMID | 12413742 |
| MeSH | Amino Acid Sequence Animals Antibodies, Monoclonal / chemistry* Antibodies, Monoclonal / immunology* Antibodies, Monoclonal / pharmacology Antibody Formation / drug effects Antibody Formation / immunology Antibody Specificity / immunology* COS Cells Humans Hybridomas / drug effects Hybridomas / immunology Interleukin-6 / immunology Interleukin-6 / pharmacology Mice Molecular Sequence Data Rats Receptors, Interleukin-6 / antagonists & inhibitors Receptors, Interleukin-6 / immunology* Species Specificity |
| IF | 3.276 |
| 引用数 | 74 |
| WOS 分野 | IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 27 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) |