Reference - Detail
| RRC ID | 41721 |
|---|---|
| Author | Asakura H, Kashio Y, Nakamura K, Seki M, Dai S, Shirato Y, Abedin MJ, Yoshida N, Nishi N, Imaizumi T, Saita N, Toyama Y, Takashima H, Nakamura T, Ohkawa M, Hirashima M. |
| Title | Selective eosinophil adhesion to fibroblast via IFN-gamma-induced galectin-9. |
| Journal | J Immunol |
| Abstract |
Among galectin family members, galectin-9 was first described as a potent eosinophil chemoattractant derived from Ag-stimulated T cells. In the present study a role of galectin-9 in the interaction between eosinophils and fibroblasts was investigated using a human lung fibroblast cell line, HFL-1. RT-PCR, real-time PCR, and Western blot analyses revealed that both galectin-9 mRNA and protein in HFL-1 cells were up-regulated by IFN-gamma stimulation. On the one hand, IL-4, known as a Th2 cytokine, did not affect the galectin-9 expression in HFL-1 cells. We further confirmed that IFN-gamma up-regulated the expression of galectin-9 in primary human dermal fibroblasts. Flow cytometric analysis revealed that IFN-gamma up-regulated surface galectin-9 expression on HFL-1 cells. Stimulation of HFL-1 cells with IFN-gamma up-regulated adhesion of eosinophils, but not neutrophils, to HFL-1 cells. This adherence of eosinophils to HFL-1 cells was inhibited by both lactose and anti-galectin-9 Ab. These findings demonstrate that IFN-gamma-induced galectin-9 expression in fibroblasts mediates eosinophil adhesion to the cells, suggesting a crucial role of galectin-9 in IFN-gamma-stimulated fibroblasts as a physiological modulator at the inflammatory sites. |
| Volume | 169(10) |
| Pages | 5912-8 |
| Published | 2002-11-15 |
| DOI | 10.4049/jimmunol.169.10.5912 |
| PMID | 12421975 |
| MeSH | Adult Cell Adhesion / physiology Cell Line Cell-Free System / chemistry Cell-Free System / physiology Chemotactic Factors, Eosinophil / biosynthesis Chemotactic Factors, Eosinophil / isolation & purification Chemotactic Factors, Eosinophil / physiology Eosinophils / physiology* Female Fibroblasts / metabolism Fibroblasts / physiology* Galectins / biosynthesis* Galectins / isolation & purification Galectins / physiology* Humans Interferon-gamma / pharmacology* Lung / metabolism Lung / pathology Lung Diseases, Interstitial / metabolism Lung Diseases, Interstitial / pathology Middle Aged Neutrophils / physiology Pulmonary Eosinophilia / metabolism Pulmonary Eosinophilia / pathology |
| IF | 4.886 |
| Times Cited | 101 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Wikipedia |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HFL-I(RCB0521) |