RRC ID 41723
Author Lung HL, Ip WK, Wong CK, Mak NK, Chen ZY, Leung KN.
Title Anti-proliferative and differentiation-inducing activities of the green tea catechin epigallocatechin-3-gallate (EGCG) on the human eosinophilic leukemia EoL-1 cell line.
Journal Life Sci
Abstract A novel approach for the treatment of leukemia is the differentiation therapy in which immature leukemia cells are induced to attain a mature phenotype when exposed to differentiation inducers, either alone or in combinations with other chemotherapeutic or chemopreventive drugs. Over the past decade, numerous studies indicated that green tea catechins (GTC) could suppress the growth and induce apoptosis on a number of human cancer cell lines. However, the differentiation-inducing activity of GTC on human tumors remains poorly understood. In the present study, the effect of the major GTC epigallocatechin-3-gallate (EGCG) on the proliferation and differentiation of a human eosinophilc leukemic cell line, EoL-1, was examined. Our results showed that EGCG suppressed the proliferation of the EoL-1 cells in a dose-dependent manner, with an estimated IC(50) value of 31.5 microM. On the other hand, EGCG at a concentration of 40 microM could trigger the EoL-1 cells to undergo morphological differentiation into mature eosinophil-like cells. Using RT-PCR and flow cytometry, it was found that EGCG upregulated the gene and protein expression of two eosinophil-specific granule proteins, the major basic protein (MBP) and eosinophil peroxidase (EPO), in EoL-1 cells. Taken together, our findings suggest that EGCG can exhibit anti-leukemic activity on a human eosinophilic cell line EoL-1 by suppressing the proliferation and by inducing the differentiation of the leukemia cells.
Volume 72(3)
Pages 257-68
Published 2002-12-6
DOI 10.1016/s0024-3205(02)02236-1
PII S0024320502022361
PMID 12427485
MeSH Antineoplastic Agents, Phytogenic / pharmacology* Blood Proteins / biosynthesis Blood Proteins / genetics Catechin / analogs & derivatives* Catechin / pharmacology* Cell Differentiation Cell Division Dose-Response Relationship, Drug Eosinophil Granule Proteins Eosinophil Peroxidase Growth Inhibitors / pharmacology Humans Hypereosinophilic Syndrome / drug therapy* Hypereosinophilic Syndrome / metabolism Hypereosinophilic Syndrome / pathology Kinetics Peroxidases / biosynthesis Peroxidases / genetics RNA, Neoplasm / biosynthesis Ribonucleases* Tumor Cells, Cultured
IF 3.647
Times Cited 40
Human and Animal Cells EoL-1(RCB0641)