論文 - 詳細
| RRC ID | 41726 |
|---|---|
| 著者 | Kihara F, Arima H, Tsutsumi T, Hirayama F, Uekama K. |
| タイトル | Effects of structure of polyamidoamine dendrimer on gene transfer efficiency of the dendrimer conjugate with alpha-cyclodextrin. |
| ジャーナル | Bioconjug Chem |
| Abstract |
To improve gene transfer activity of a new nonviral vector, a polyamidoamine dendrimer (G2) conjugate with alpha-cyclodextrin (alpha-CDE conjugate (G2)), we prepared alpha-CDE conjugates with dendrimer having different generations (G3 and G4), and their gene transfer activities were compared with those of alpha-CDE conjugate (G2) and TransFast, a novel transfection reagent. alpha-CDE conjugates (G2, G3, and G4) formed the complexes with pDNA, changing the zeta-potential and particle size of pDNA complexes and the protection of pDNA from DNase I in a charge ratio-dependent manner, although their differences at higher charge ratios (vector/pDNA) were small. The gene transfer activity of alpha-CDE conjugates (G2, G3, and G4) was higher than that of the corresponding dendrimer alone in NIH3T3 and RAW264.7 cells. Of these CDE conjugates, alpha-CDE conjugate (G3) had a superior gene transfer activity which was comparable to that of TransFast in NIH3T3 cells. The intracellular distribution of pDNA after application of the pDNA complex with alpha-CDE conjugate (G3) to NIH3T3 cells was different from that with dendrimer alone (G3), although the cellular association of pDNA was almost comparable among all vectors. alpha-CDE conjugate (G3) strongly interacted with a fluorescence probe, 2-(p-toluidinyl)-naphthalene-6-sulfonate (TNS), suggesting that the conjugate possesses the inclusion ability with biomembrane constituents such as phospholipids after transfection. These results suggest that alpha-CDE conjugates, particularly the G3 conjugate, could be novel nonviral gene transfer agents. |
| 巻・号 | 13(6) |
| ページ | 1211-9 |
| 公開日 | 2002-1-1 |
| DOI | 10.1021/bc025557d |
| PII | bc025557d |
| PMID | 12440855 |
| MeSH | 3T3 Cells Animals Biological Transport Cyclodextrins / adverse effects Cyclodextrins / chemistry* DNA / metabolism* Deoxyribonuclease I / metabolism Drug Carriers / adverse effects Drug Carriers / chemistry* Gene Expression Mice Particle Size Polymers / adverse effects Polymers / chemistry* Static Electricity Transfection / methods* Tumor Cells, Cultured alpha-Cyclodextrins* |
| IF | 4.031 |
| 引用数 | 121 |
| WOS 分野 | CHEMISTRY, ORGANIC BIOCHEMICAL RESEARCH METHODS CHEMISTRY, MULTIDISCIPLINARY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |