| RRC ID |
41727
|
| 著者 |
Goseki-Sone M, Yamada A, Hamatani R, Mizoi L, Iimura T, Ezawa I.
|
| タイトル |
Phosphate depletion enhances bone morphogenetic protein-4 gene expression in a cultured mouse marrow stromal cell line ST2.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Alkaline phosphatases (ALPs) are glycosylated, membrane-bound enzymes that hydrolyze various monophosphate esters at an optimum high pH and are present in nearly all living organisms. In Escherichia coli, extracellular phosphate (Pi) limitation induces the ALP gene, indicating a role of extracellular Pi in ALP gene regulation. However, little is known about similar mechanisms in mammalian cells. Previously, we reported that Pi starvation increased the tissue-nonspecific ALP (TNSALP) activity and regulated its expression in the mouse stromal cell line ST2, derived from mouse bone marrow. In the present study, we further examined the effects of Pi starvation on the mechanism of TNSALP induction. The specific activity of TNSALP increased markedly after treatment by Pi starvation for 5 days and RT-PCR analysis revealed that the mRNA of the bone morphogenetic protein-4 (BMP-4) gene was highly stimulated. The combination of Pi depletion and mouse BMP-4 receptor IA/Fc chimera down-regulated the TNSALP activity. These results indicated that Pi depletion stimulates the TNSALP activity for the Pi supplementation, and that this system may involve the signaling pathway of the BMP-4 gene at the transcription level.
|
| 巻・号 |
299(3)
|
| ページ |
395-9
|
| 公開日 |
2002-12-6
|
| DOI |
10.1016/s0006-291x(02)02646-3
|
| PII |
S0006291X02026463
|
| PMID |
12445813
|
| MeSH |
Alkaline Phosphatase / genetics
Alkaline Phosphatase / metabolism*
Animals
Bone Marrow Cells / cytology
Bone Marrow Cells / physiology*
Bone Morphogenetic Protein 4
Bone Morphogenetic Proteins / genetics*
Bone Morphogenetic Proteins / metabolism
Cell Line
Gene Expression Regulation
Humans
Mice
Phosphates / metabolism*
Recombinant Fusion Proteins / metabolism
Signal Transduction / physiology
Stromal Cells / cytology
Stromal Cells / physiology*
Transcription, Genetic
|
| IF |
2.985
|
| 引用数 |
14
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
ST2(RCB0224) |
