| RRC ID |
41738
|
| 著者 |
den Engelsman J, Keijsers V, de Jong WW, Boelens WC.
|
| タイトル |
The small heat-shock protein alpha B-crystallin promotes FBX4-dependent ubiquitination.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
AlphaB-crystallin is a small heat-shock protein in which three serine residues (positions 19, 45, and 59) can be phosphorylated under various conditions. We describe here the interaction of alphaB-crystallin with FBX4, an F-box-containing protein that is a component of the ubiquitin-protein isopeptide ligase SCF (SKP1/CUL1/F-box). The interaction with FBX4 was enhanced by mimicking phosphorylation of alphaB-crystallin at both Ser-19 and Ser-45 (S19D/S45D), but not at other combinations. Ser-19 and Ser-45 are preferentially phosphorylated during the mitotic phase of the cell cycle. Also alphaB-crystallin R120G, a mutant found to co-segregate with a desmin-related myopathy, displayed increased interaction with FBX4. Both alphaB-crystallin S19D/S45D and R120G specifically translocated FBX4 to the detergent-insoluble fraction and stimulated the ubiquitination of one or a few yet unknown proteins. These findings implicate the involvement of alphaB-crystallin in the ubiquitin/proteasome pathway in a phosphorylation- and cell cycle-dependent manner and may provide new insights into the alphaB-crystallin-induced aggregation in desmin-related myopathy.
|
| 巻・号 |
278(7)
|
| ページ |
4699-704
|
| 公開日 |
2003-2-14
|
| DOI |
10.1074/jbc.M211403200
|
| PII |
S0021-9258(19)32766-8
|
| PMID |
12468532
|
| MeSH |
Cell Cycle / physiology
HeLa Cells
Humans
Mutagenesis, Site-Directed
Peptide Synthases / metabolism*
Protein Binding
SKP Cullin F-Box Protein Ligases
Signal Transduction
Ubiquitin / metabolism*
alpha-Crystallin B Chain / genetics
alpha-Crystallin B Chain / metabolism*
|
| IF |
4.238
|
| 引用数 |
105
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
anti-αB-crystallin(RCB1304) |
