論文 - 詳細
| RRC ID | 41738 |
|---|---|
| 著者 | den Engelsman J, Keijsers V, de Jong WW, Boelens WC. |
| タイトル | The small heat-shock protein alpha B-crystallin promotes FBX4-dependent ubiquitination. |
| ジャーナル | J Biol Chem |
| Abstract |
AlphaB-crystallin is a small heat-shock protein in which three serine residues (positions 19, 45, and 59) can be phosphorylated under various conditions. We describe here the interaction of alphaB-crystallin with FBX4, an F-box-containing protein that is a component of the ubiquitin-protein isopeptide ligase SCF (SKP1/CUL1/F-box). The interaction with FBX4 was enhanced by mimicking phosphorylation of alphaB-crystallin at both Ser-19 and Ser-45 (S19D/S45D), but not at other combinations. Ser-19 and Ser-45 are preferentially phosphorylated during the mitotic phase of the cell cycle. Also alphaB-crystallin R120G, a mutant found to co-segregate with a desmin-related myopathy, displayed increased interaction with FBX4. Both alphaB-crystallin S19D/S45D and R120G specifically translocated FBX4 to the detergent-insoluble fraction and stimulated the ubiquitination of one or a few yet unknown proteins. These findings implicate the involvement of alphaB-crystallin in the ubiquitin/proteasome pathway in a phosphorylation- and cell cycle-dependent manner and may provide new insights into the alphaB-crystallin-induced aggregation in desmin-related myopathy. |
| 巻・号 | 278(7) |
| ページ | 4699-704 |
| 公開日 | 2003-2-14 |
| DOI | 10.1074/jbc.M211403200 |
| PII | S0021-9258(19)32766-8 |
| PMID | 12468532 |
| MeSH | Cell Cycle / physiology HeLa Cells Humans Mutagenesis, Site-Directed Peptide Synthases / metabolism* Protein Binding SKP Cullin F-Box Protein Ligases Signal Transduction Ubiquitin / metabolism* alpha-Crystallin B Chain / genetics alpha-Crystallin B Chain / metabolism* |
| IF | 4.238 |
| 引用数 | 105 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | anti-αB-crystallin(RCB1304) |