RRC ID 41755
著者 Fujita J, Ohtsuki Y, Bandoh S, Ueda Y, Kubo A, Tojo Y, Yamaji Y, Ishida T.
タイトル Expression of thyroid transcription factor-1 in 16 human lung cancer cell lines.
ジャーナル Lung Cancer
Abstract It has been suggested that thyroid transcription factor-1 (TTF-1) is frequently expressed in human lung cancer, especially in adenocarcinoma and small cell lung cancer, and the TTF-1 expression is closely related with the expression of surfactant protein. We hypothesized that TTF-1 is expressed in human lung cancer cell lines and its expression might be related to the expression of surfactant protein. To test this, expressions of TTF-1 and surfactant protein A (SP-A) were immunohistochemically evaluated in 16 human lung cancer cell lines. In addition, expressions of mRNAs for TTF-1 and SP-A were analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) and sequencing. As a result, nuclear staining of TTF-1 was observed in two of six adenocarcinoma cell lines, none of seven small cell lung cancer cell lines, and none of three squamous lung cancer cell lines. Among the 16 cell lines, six cell lines (PC3, LC2/Ad, A549, RERF-LC-OK, HI1017, and PC9) expressed significant amounts of mRNA for TTF-1. In contrast, cytoplasmic staining of TTF-1 was observed in five of six adenocarcinoma cell lines, in six of seven small cell lung cancer cell lines, and in all three squamous cell lung cancer cell lines. One of the two adenocarcinoma cell lines those showed positive nuclear staining and cytoplasmic SP-A staining released a significant amount of SP-A in culture supernatant. Our present study demonstrates that the frequency of TTF-1 expression in the nucleus was very low in human lung cancer cell lines; however, their cytoplasmic positivities should be further investigated.
巻・号 39(1)
ページ 31-6
公開日 2003-1-1
DOI 10.1016/s0169-5002(02)00390-2
PII S0169500202003902
PMID 12499091
MeSH Adenocarcinoma / genetics Adenocarcinoma / metabolism Carcinoma, Small Cell / genetics Carcinoma, Small Cell / metabolism Carcinoma, Squamous Cell / genetics Carcinoma, Squamous Cell / metabolism Culture Media, Conditioned / chemistry Gene Expression Regulation, Neoplastic* Humans Immunohistochemistry Lung Neoplasms / genetics Lung Neoplasms / metabolism* Nuclear Proteins / genetics Nuclear Proteins / metabolism* Pulmonary Surfactant-Associated Protein A / genetics Pulmonary Surfactant-Associated Protein A / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction Thyroid Nuclear Factor 1 Transcription Factors / genetics Transcription Factors / metabolism* Tumor Cells, Cultured
IF 4.702
引用数 19
WOS 分野 RESPIRATORY SYSTEM ONCOLOGY
リソース情報
ヒト・動物細胞