| RRC ID |
41764
|
| 著者 |
Misawa E, Sakurai T, Yamada M, Hayasawa H, Motoyoshi K.
|
| タイトル |
Booster effect of interleukin-2 on natural killer 1.1+ cells stimulated by administration of macrophage colony-stimulating factor in mice.
|
| ジャーナル |
J Immunother
|
| Abstract |
The authors studied the combined effects of macrophage colony-stimulating factor (M-CSF) and interleukin (IL)-2 on the functions and antitumor activity of natural killer (NK) 1.1+ cells in vitro and in vivo. NK1.1+ cells were isolated from the spleen of mice treated with saline or M-CSF, and their functions (proliferation, production of IFN-gamma, and cytotoxicity) evaluated in vitro. Although the proliferation of and production by NK1.1+ cells was stimulated by the addition of IL-2, the cells from the M-CSF-treated mice responded better. Furthermore, the cytotoxicity against Yac-1 cells and B16 melanoma cells was stimulated by M-CSF administration and enhanced by the addition of IL-2 and IL-12. These results demonstrated that M-CSF treatment augmented the functions of NK1.1 cells, and IL-2 and IL-12 boosted these activities in vitro. The authors then examined the effects of co-administration of M-CSF and IL-2 in vivo. The clearance of B16 cells in lung was augmented by the administration of M-CSF but not IL-2. However, M-CSF + IL-2 treatment further enhanced the clearance activity. The anti-metastatic activity was also enhanced by the M-CSF + IL-2 treatment. Furthermore, the survival of B16-bearing mice was prolonged by M-CSF + IL-2. These results suggested that administration of IL-2 boosts the functions of NK1.1+ cells, which are augmented preliminarily by the administration of M-CSF.
|
| 巻・号 |
26(1)
|
| ページ |
21-30
|
| 公開日 |
2003-1-1
|
| DOI |
10.1097/00002371-200301000-00003
|
| PMID |
12514426
|
| MeSH |
Animals
Antigens / immunology
Antigens, Ly
Antigens, Surface
Cell Division / physiology
Cytotoxicity, Immunologic / drug effects
Disease Models, Animal
Drug Interactions
Interferon-gamma / analysis
Interferon-gamma / biosynthesis*
Interleukin-2 / pharmacology*
Killer Cells, Natural / drug effects*
Killer Cells, Natural / immunology
Lectins, C-Type
Lymphocyte Activation
Macrophage Colony-Stimulating Factor / pharmacology*
Male
Melanoma, Experimental
Mice
Mice, Inbred C57BL
NK Cell Lectin-Like Receptor Subfamily B
Proteins / immunology
Random Allocation
Sensitivity and Specificity
|
| IF |
4.11
|
| 引用数 |
5
|
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
IMMUNOLOGY
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
YAC-1(RCB1165)
B16 melanoma |
