Reference - Detail
| RRC ID | 41771 |
|---|---|
| Author | Kawakita Y, Ikekita M, Kurozumi R, Kojima S. |
| Title | Increase of intracellular glutathione by low-dose gamma-ray irradiation is mediated by transcription factor AP-1 in RAW 264.7 cells. |
| Journal | Biol Pharm Bull |
| Abstract |
The mechanism of the elevation of intracellular glutathione induced by low-dose gamma-rays was examined in RAW 264.7 cells. The expression of mRNA for gamma-glutamylcysteine synthetase (gamma-GCS) increased soon after gamma-ray (0.5 Gy) irradiation, and peaked between 3 h and 6 h post-irradiation. A dose of 0.25 to 0.5 Gy was optimum for induction of gamma-GCS mRNA expression at 3 h post-irradiation. The effect of inhibitors of activator protein-1 (AP-1) and nuclear factor kappaB (NF-kappaB) on the radiation-induced gamma-GCS gene expression was then examined. The induction of gamma-GCS mRNA expression was significantly suppressed when AP-1 DNA binding, but not NF-kappaB DNA binding, was inhibited. Finally, electrophoretic mobility shift assay showed that the low-dose radiation markedly increased the DNA binding of AP-1, but not NF-kappaB, soon after irradiation. These results suggest that the increase of glutathione levels in RAW 264.7 cells by low-dose gamma-ray irradiation is mediated by transcriptional regulation of the gamma-GCS gene, predominantly through the AP-1 binding site in its promoter. |
| Volume | 26(1) |
| Pages | 19-23 |
| Published | 2003-1-1 |
| DOI | 10.1248/bpb.26.19 |
| PMID | 12520165 |
| MeSH | Animals Cell Line Dose-Response Relationship, Radiation Gamma Rays* Gene Expression Regulation / physiology Gene Expression Regulation / radiation effects Glutathione / biosynthesis* Glutathione / radiation effects* Intracellular Fluid / metabolism Intracellular Fluid / radiation effects* Mice Transcription Factor AP-1 / physiology* Transcription Factor AP-1 / radiation effects* |
| IF | 1.863 |
| Times Cited | 29 |
| WOS Category | PHARMACOLOGY & PHARMACY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RAW 264(RCB0535) |