| RRC ID |
41777
|
| 著者 |
Hagihara M, Tsuchiya T, Hyodo O, Ueda Y, Tazume K, Gansuvd B, Kato S, Hotta T.
|
| タイトル |
The in vitro generation of Ph1+ ALL-specific HLA-A24-restricted cytotoxic T lymphocytes using a synthetic 16 mer minor bcr-abl peptide.
|
| ジャーナル |
Leuk Res
|
| Abstract |
Sixteen mer peptide, which spans the junctional region of the acute lymphoid leukemia (ALL)-specific minor bcr-abl fusion protein and contains a motif that can bind to human leukocyte antigen (HLA)-A24, was constructed. We tried to generate Philadelphia chromosome 1 (Ph1) positive ALL-specific cytotoxic T lymphocytes (CTLs) from eight normal HLA-A24+ individuals with peptide-pulsed autologous dendritic cells (DCs). CTLs could be generated from the mononuclear cells (MNCs) of a single donor, which could kill peptide-pulsed autologous DCs and two A24+ ALL lines, while an HLA-A24+ CML line was only weakly killed and unpulsed DCs or the control lines Daudi or K562 were not recognized. Those CTLs consisted predominantly of CD8+ T cells whose cytotoxicity could be neutralized by monoclonal antibodies to HLA-class I or HLA-A24, and also produced interferon (IFN)-gamma after being stimulated with peptide-pulsed DCs.
|
| 巻・号 |
27(3)
|
| ページ |
253-7
|
| 公開日 |
2003-3-1
|
| DOI |
10.1016/s0145-2126(02)00092-9
|
| PII |
S0145212602000929
|
| PMID |
12537978
|
| MeSH |
Antigen Presentation / immunology
CD8-Positive T-Lymphocytes / immunology
CD8-Positive T-Lymphocytes / metabolism
Coculture Techniques
Cytotoxicity, Immunologic
Dendritic Cells / immunology
Fusion Proteins, bcr-abl / immunology*
HLA-A Antigens / immunology*
HLA-A24 Antigen
Humans
Interferon-gamma / metabolism
Killer Cells, Lymphokine-Activated / immunology
Oligopeptides / chemical synthesis
Oligopeptides / immunology*
Precursor Cell Lymphoblastic Leukemia-Lymphoma / immunology*
Precursor Cell Lymphoblastic Leukemia-Lymphoma / pathology
T-Lymphocytes, Cytotoxic / cytology
T-Lymphocytes, Cytotoxic / immunology*
Tumor Cells, Cultured
|
| IF |
2.214
|
| 引用数 |
3
|
|
WOS 分野
|
HEMATOLOGY
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
