論文 - 詳細
| RRC ID | 41777 |
|---|---|
| 著者 | Hagihara M, Tsuchiya T, Hyodo O, Ueda Y, Tazume K, Gansuvd B, Kato S, Hotta T. |
| タイトル | The in vitro generation of Ph1+ ALL-specific HLA-A24-restricted cytotoxic T lymphocytes using a synthetic 16 mer minor bcr-abl peptide. |
| ジャーナル | Leuk Res |
| Abstract |
Sixteen mer peptide, which spans the junctional region of the acute lymphoid leukemia (ALL)-specific minor bcr-abl fusion protein and contains a motif that can bind to human leukocyte antigen (HLA)-A24, was constructed. We tried to generate Philadelphia chromosome 1 (Ph1) positive ALL-specific cytotoxic T lymphocytes (CTLs) from eight normal HLA-A24+ individuals with peptide-pulsed autologous dendritic cells (DCs). CTLs could be generated from the mononuclear cells (MNCs) of a single donor, which could kill peptide-pulsed autologous DCs and two A24+ ALL lines, while an HLA-A24+ CML line was only weakly killed and unpulsed DCs or the control lines Daudi or K562 were not recognized. Those CTLs consisted predominantly of CD8+ T cells whose cytotoxicity could be neutralized by monoclonal antibodies to HLA-class I or HLA-A24, and also produced interferon (IFN)-gamma after being stimulated with peptide-pulsed DCs. |
| 巻・号 | 27(3) |
| ページ | 253-7 |
| 公開日 | 2003-3-1 |
| DOI | 10.1016/s0145-2126(02)00092-9 |
| PII | S0145212602000929 |
| PMID | 12537978 |
| MeSH | Antigen Presentation / immunology CD8-Positive T-Lymphocytes / immunology CD8-Positive T-Lymphocytes / metabolism Coculture Techniques Cytotoxicity, Immunologic Dendritic Cells / immunology Fusion Proteins, bcr-abl / immunology* HLA-A Antigens / immunology* HLA-A24 Antigen Humans Interferon-gamma / metabolism Killer Cells, Lymphokine-Activated / immunology Oligopeptides / chemical synthesis Oligopeptides / immunology* Precursor Cell Lymphoblastic Leukemia-Lymphoma / immunology* Precursor Cell Lymphoblastic Leukemia-Lymphoma / pathology T-Lymphocytes, Cytotoxic / cytology T-Lymphocytes, Cytotoxic / immunology* Tumor Cells, Cultured |
| IF | 2.214 |
| 引用数 | 3 |
| WOS 分野 | HEMATOLOGY ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | K562(RCB0027) |