Reference - Detail
| RRC ID | 4177 |
|---|---|
| Author | McMiller TL, Sims D, Lee T, Williams T, Johnson CM. |
| Title | Molecular characterization of the Caenorhabditis elegans REF-1 family member, hlh-29/hlh-28. |
| Journal | Biochim Biophys Acta |
| Abstract |
Members of the Caenorhabditis elegans REF-1 family of bHLH proteins are atypical in that each protein contains two bHLH domains. In this study we describe a functional and molecular characterization of the REF-1 family members, hlh-29/hlh-28. 5'-RACE results confirm the presence of two bHLH domain coding regions in a single transcript and quantitative PCR (qPCR) shows that hlh-29/hlh-28 mRNA is detected in wild-type animals throughout development. A promoter fusion of hlh-29 to the green fluorescent protein shows post-embryonic reporter activity in cells of the vulva, the somatic gonad, the intestine and in neuronal cells of the head and tail. Loss of hlh-29/hlh-28 function via RNA interference (RNAi) results in multiple phenotypes including late embryonic lethality, yolk protein accumulation, everted vulva, bordering behavior, and alter chemosensory responses. |
| Volume | 1769(1) |
| Pages | 5-19 |
| Published | 2007-1-1 |
| DOI | 10.1016/j.bbaexp.2006.12.001 |
| PII | S0167-4781(06)00208-9 |
| PMID | 17258327 |
| MeSH | Animals Base Sequence Basic Helix-Loop-Helix Transcription Factors / chemistry Basic Helix-Loop-Helix Transcription Factors / genetics* Basic Helix-Loop-Helix Transcription Factors / pharmacology Basic Helix-Loop-Helix Transcription Factors / physiology* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / pharmacology Caenorhabditis elegans Proteins / physiology* Embryo, Nonmammalian Embryonic Development / genetics Female Gene Expression Regulation, Developmental Gonads / embryology Gonads / metabolism Molecular Sequence Data Neurons / metabolism RNA Interference Tissue Distribution Vulva / embryology Vulva / metabolism |
| IF | 3.411 |
| Times Cited | 5 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| C.elegans | tm458 tm284 |