論文 - 詳細
| RRC ID | 41795 |
|---|---|
| 著者 | Isobe K, Tatsuno I, Yashiro T, Nanmoku T, Takekoshi K, Kawakami Y, Nakai T. |
| タイトル | Expression of mRNA for PACAP and its receptors in intra- and extra-adrenal human pheochromocytomas and their relationship to catecholamine synthesis. |
| ジャーナル | Regul Pept |
| Abstract |
PURPOSE:Pituitary adenylate cyclase-activating polypeptide (PACAP), a member of the secretin/glucagons/vasoactive intestinal peptide family, induces the expression of catecholamine-synthesizing enzymes in adrenal medullary cells. In addition, PACAP and its receptor have been detected in human pheochromocytoma tissues, though it is not yet known whether PACAP enhances the expression of genes encoding catecholamine-synthesizing enzymes. To address this question, we analyzed PACAP, PACAP receptor, and tyrosine hydroxylase (TH) and phenylethanolamine-N-methyltransferase (PNMT) mRNAs in pheochromocytomas. METHODS:The levels of the mRNA for PACAP and vasoactive intestinal peptide (VIP), and their receptors, and for TH and PNMT were measured by RT-PCR or real-time PCR analysis, and the concentrations of catecholamines were measured by HPLC in 24 intra-adrenal and six extra-adrenal pheochromocytomas. RESULTS:mRNA expression of PACAP and its receptor VPAC1R were detected in many pheochromocytomas (24/30 and 29/30, respectively), but mRNA expression of the PAC1R and VPAC2R receptor subtypes were detected in only one of six extra-adrenal pheochromocytomas. PACAP mRNA expression correlated with TH (p=0.0018) and PNMT (p=0.05) mRNA expression, as well as epinephrine (p=0.0342) levels in 16 intra-adrenal pheochromocytomas. CONCLUSION:Our findings support a possible role for PACAP in the regulation of expression of genes encoding catecholamine-synthesizing enzymes in intra-adrenal pheochromocytomas. |
| 巻・号 | 110(3) |
| ページ | 213-7 |
| 公開日 | 2003-2-28 |
| DOI | 10.1016/s0167-0115(02)00209-4 |
| PII | S0167011502002094 |
| PMID | 12573802 |
| MeSH | Adrenal Gland Neoplasms / genetics* Adrenal Gland Neoplasms / metabolism Catecholamines / biosynthesis* Cell Line, Tumor Gene Expression Regulation, Neoplastic* Humans Neuropeptides / genetics* Pheochromocytoma / genetics* Pheochromocytoma / metabolism Pituitary Adenylate Cyclase-Activating Polypeptide RNA, Messenger / genetics RNA, Messenger / metabolism Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I Receptors, Pituitary Hormone / genetics* Reverse Transcriptase Polymerase Chain Reaction |
| 引用数 | 22 |
| WOS 分野 | PHYSIOLOGY ENDOCRINOLOGY & METABOLISM |
| リソース情報 | |
| ヒト・動物細胞 | CHP-134(RCB0487) |