RRC ID |
41797
|
著者 |
Kurihara K, Shingo Y, Miura NN, Horie S, Usui Y, Adachi Y, Yadomae T, Ohno N.
|
タイトル |
Effect of CAWS, a mannoprotein-beta-glucan complex of Candida albicans, on leukocyte, endothelial cell, and platelet functions in vitro.
|
ジャーナル |
Biol Pharm Bull
|
Abstract |
Candida albicans is a medically important fungus which induces a disseminated candidasis and candidemia in immunocompromised hosts, and releases a polysaccharide fraction into the blood. We recently found that C. albicans released a water-soluble polysaccharide fraction (CAWS) into synthetic medium and demonstrated that CAWS was mainly composed of a complex of mannan and beta-glucan. In the murine system, CAWS showed a lethality resembling anaphylactic shock when administered i.v., and induced coronary arteritis similar to Kawasaki Disease (KD) when given i.p. In the present study, we examined the biological activity of CAWS in the cell culture and found the following: i) CAWS slightly induced production of IFN-gamma and IL-6 by splenocytes at lower dose (ca. 10 micro g/ml), but at a higher dose strongly inhibited the proliferation of splenocytes induced by a B cell mitogen, lipopolysaccharide (LPS) and a T cell mitogen, concanavalin A. ii) The viability of these splenocytes monitored by propidium iodide staining was significantly reduced. iii) The addition of CAWS to a culture of monophage RAW264.7 cells significantly reduced cellular growth rate dose dependently. iv) The LPS-mediated synthesis of cytokines by RAW264.7 cells was significantly inhibited by CAWS. v) CAWS induced an aggregation of platelets in human platelet-rich plasma, and vi) CAWS inhibited the production of thrombomodulin by human umbilical endothelial cells and acted synergistically with TNF-alpha. Thus, CAWS strongly inhibited the cellular functions of leukocytes in vitro, partly through direct cytotoxicity. The enhanced production in injured cells of the vascular endothelium would be related to the local inflammatory response in the coronary artery.
|
巻・号 |
26(2)
|
ページ |
233-40
|
公開日 |
2003-2-1
|
DOI |
10.1248/bpb.26.233
|
PMID |
12576686
|
MeSH |
Animals
Blood Platelets / cytology
Blood Platelets / drug effects*
Blood Platelets / physiology
Candida albicans* / chemistry
Candida albicans* / isolation & purification
Cell Line
Endothelium, Vascular / cytology
Endothelium, Vascular / drug effects*
Endothelium, Vascular / physiology
Glucans / chemistry
Glucans / isolation & purification
Glucans / pharmacology*
Humans
Leukocytes / cytology
Leukocytes / drug effects*
Leukocytes / physiology
Male
Membrane Glycoproteins / chemistry
Membrane Glycoproteins / isolation & purification
Membrane Glycoproteins / pharmacology*
Mice
Mice, Inbred C3H
Mice, Inbred DBA
|
IF |
1.863
|
引用数 |
30
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
|
リソース情報 |
ヒト・動物細胞 |
RAW 264(RCB0535) |