RRC ID 41814
著者 Aramaki Y, Lee I, Arima H, Sakamoto T, Magami Y, Yoshimoto T, Moriyasu F, Mizuguchi J, Koyanagi Y, Nikaido T, Tsuchiya S.
タイトル Efficient gene transfer to hepatoblastoma cells through asialoglycoprotein receptor and expression under the control of the cyclin A promoter.
ジャーナル Biol Pharm Bull
Abstract Specific gene delivery into hepatoma cells by liposomes and specific gene expression under the control of the cyclin A promoter were examined in HepG2 cells, a hepatoblastoma cell line that overexpresses cyclin A. A plasmid carrying the luciferase gene under the cyclin A promoter sequence was condensed with poly-L-lysine and encapsulated into anionic asialofetuin-labeled liposomes (AF-liposomes), which were preferentially taken up by hepatocytes through the action of the asialoglycoprotein receptor (AgpR). AF-liposomes delivered plasmids to the hepatoma cells by receptor-mediated endocytosis through the AgpR, and transgene expression could be achieved under the control of the cyclin A promoter. Furthermore, a fusogenic lipid, DOPE, as a liposomal component was required for the enhancement of transfection efficiency of AF-liposomes.
巻・号 26(3)
ページ 357-60
公開日 2003-3-1
DOI 10.1248/bpb.26.357
PMID 12612447
MeSH Antimalarials / pharmacology Asialoglycoprotein Receptor / metabolism* Cell Line, Tumor Chloroquine / pharmacology Cyclin A / genetics* Gene Expression Gene Transfer Techniques* Hepatoblastoma / pathology Humans Liposomes / chemistry Liposomes / metabolism Luciferases / genetics Membrane Fusion Phosphatidylethanolamines / pharmacology Plasmids / metabolism Polylysine / metabolism Promoter Regions, Genetic Transfection / methods*
IF 1.863
引用数 10
WOS 分野 PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞 Hep G2