論文 - 詳細
| RRC ID | 41814 |
|---|---|
| 著者 | Aramaki Y, Lee I, Arima H, Sakamoto T, Magami Y, Yoshimoto T, Moriyasu F, Mizuguchi J, Koyanagi Y, Nikaido T, Tsuchiya S. |
| タイトル | Efficient gene transfer to hepatoblastoma cells through asialoglycoprotein receptor and expression under the control of the cyclin A promoter. |
| ジャーナル | Biol Pharm Bull |
| Abstract |
Specific gene delivery into hepatoma cells by liposomes and specific gene expression under the control of the cyclin A promoter were examined in HepG2 cells, a hepatoblastoma cell line that overexpresses cyclin A. A plasmid carrying the luciferase gene under the cyclin A promoter sequence was condensed with poly-L-lysine and encapsulated into anionic asialofetuin-labeled liposomes (AF-liposomes), which were preferentially taken up by hepatocytes through the action of the asialoglycoprotein receptor (AgpR). AF-liposomes delivered plasmids to the hepatoma cells by receptor-mediated endocytosis through the AgpR, and transgene expression could be achieved under the control of the cyclin A promoter. Furthermore, a fusogenic lipid, DOPE, as a liposomal component was required for the enhancement of transfection efficiency of AF-liposomes. |
| 巻・号 | 26(3) |
| ページ | 357-60 |
| 公開日 | 2003-3-1 |
| DOI | 10.1248/bpb.26.357 |
| PMID | 12612447 |
| MeSH | Antimalarials / pharmacology Asialoglycoprotein Receptor / metabolism* Cell Line, Tumor Chloroquine / pharmacology Cyclin A / genetics* Gene Expression Gene Transfer Techniques* Hepatoblastoma / pathology Humans Liposomes / chemistry Liposomes / metabolism Luciferases / genetics Membrane Fusion Phosphatidylethanolamines / pharmacology Plasmids / metabolism Polylysine / metabolism Promoter Regions, Genetic Transfection / methods* |
| IF | 1.863 |
| 引用数 | 10 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2 |