論文 - 詳細
RRC ID | 41816 |
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著者 | Murata T, Sugatani T, Shimizu K, Manganiello VC, Tagawa T. |
タイトル | Effect of the phosphodiesterase 4 inhibitor, rolipram, on retinoic acid-increased alkaline phosphatase activity in the mouse fibroblastic C3H10T1/2 cell line. |
ジャーナル | Arch Oral Biol |
Abstract |
We have evaluated effects of a phosphodiesterase (PDE) 4 inhibitor on retinoic acid-increased alkaline phosphatase activity in the mouse fibroblastic C3H10T1/2 clone 8 (10T1/2) cell line. 10T1/2 cells were cultured in minimum essential medium (MEM) and 10% fetal bovine serum with or without 1 microM retinoic acid and/or the PDE 4 inhibitor, rolipram, and harvested at specific intervals before measurement of alkaline phosphatase activity, cAMP production in response to parathyroid hormone, osteocalcin synthesis and expression, and phosphodiesterase activity. Retinoic acid-increased alkaline phosphatase activity, and slightly enhanced cAMP production in response to parathyroid hormone. However, it did not affect osteocalcin synthesis and expression. In the presence of retinoic acid, PDE 4 activity was not changed. A PDE 4 inhibitor, rolipram, and cAMP analog, 8-bromo-cAMP dramatically increased retinoic acid's ability to induce alkaline phosphatase activity. This is the first report that PDE 4 may be involved in regulation of retinoic acid-increased alkaline phosphatase activity. |
巻・号 | 48(1) |
ページ | 63-7 |
公開日 | 2003-1-1 |
DOI | 10.1016/s0003-9969(02)00166-8 |
PII | S0003996902001668 |
PMID | 12615143 |
MeSH | 3',5'-Cyclic-AMP Phosphodiesterases / antagonists & inhibitors 3',5'-Cyclic-AMP Phosphodiesterases / metabolism 3',5'-Cyclic-AMP Phosphodiesterases / physiology Alkaline Phosphatase / drug effects* Alkaline Phosphatase / metabolism Animals Cell Line Cyclic AMP / biosynthesis Cyclic Nucleotide Phosphodiesterases, Type 4 Drug Synergism Fibroblasts / drug effects* Fibroblasts / enzymology Mice Osteocalcin / biosynthesis Osteocalcin / genetics Parathyroid Hormone / pharmacology Phosphodiesterase Inhibitors / pharmacology* Rolipram / pharmacology* Tretinoin / pharmacology* |
IF | 1.931 |
引用数 | 1 |
WOS 分野 | DENTISTRY, ORAL SURGERY & MEDICINE |
リソース情報 | |
ヒト・動物細胞 | 10T1/2(RCB0247) MC3T3-E1(RCB1126) |