論文 - 詳細
| RRC ID | 41824 |
|---|---|
| 著者 | Noguchi S, Hirashima N, Furuno T, Nakanishi M. |
| タイトル | Remarkable induction of apoptosis in cancer cells by a novel cationic liposome complexed with a bcl-2 antisense oligonucleotide. |
| ジャーナル | J Control Release |
| Abstract |
We reported recently a novel cationic cholesterol derivative with a hydroxyethylamino head group, cholesteryl-3beta-carboxyamidoethylene-N-hydroxyethylamine (I) for liposome-mediated gene transfection [FEBS Lett., 408 (1997) 232]. In the present paper we have studied whether this novel cationic liposome is prominent in nature to suppress cell growth of human cancer cells. Bcl-2 antisense phosphorothioate oligonucleotides (AS-ODNs) were complexed with the cationic liposomes with the derivative (I) and they were introduced into human cervix epithelial carcinoma cell lines HeLa, and mouse fibroblast NIH3T3 cells. An AS-ODNs targeting/bcl-2 gene induced probably apoptosis (including necrosis in some cases) in HeLa and NIH3T3 cells, however, nonsense oligonucleotides (NS-ODNs) corresponding to a scrambled-sequence control hardly induced apoptosis. Induction of apoptosis was much greater than that by commercially available DC-Chol liposomes. Fluorescence intensities of FITC-conjugated bcl-2 AS-ODNs were specifically found in the nucleus. The intensity of the AS-ODNs was mostly consistent with the amounts of Bcl-2 proteins observed by Western blot analysis in the target cells. The results showed the possibility that this new cationic cholesterol derivative might be very promising to be used for liposome-mediated gene targeting in vitro and in vivo. |
| 巻・号 | 88(2) |
| ページ | 313-20 |
| 公開日 | 2003-3-7 |
| DOI | 10.1016/s0168-3659(02)00484-4 |
| PII | S0168365902004844 |
| PMID | 12628337 |
| MeSH | Apoptosis / drug effects* Cations DNA, Neoplasm / biosynthesis DNA, Neoplasm / genetics Flow Cytometry Genes, Reporter / genetics Genes, bcl-2 / genetics* Humans Immunoblotting Liposomes Luciferases Microscopy, Confocal Neoplasms / pathology* Oligonucleotides, Antisense / administration & dosage* Oligonucleotides, Antisense / pharmacology* Tumor Cells, Cultured |
| IF | 7.727 |
| 引用数 | 13 |
| WOS 分野 | CHEMISTRY, MULTIDISCIPLINARY PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) NIH3T3 |