RRC ID 41824
著者 Noguchi S, Hirashima N, Furuno T, Nakanishi M.
タイトル Remarkable induction of apoptosis in cancer cells by a novel cationic liposome complexed with a bcl-2 antisense oligonucleotide.
ジャーナル J Control Release
Abstract We reported recently a novel cationic cholesterol derivative with a hydroxyethylamino head group, cholesteryl-3beta-carboxyamidoethylene-N-hydroxyethylamine (I) for liposome-mediated gene transfection [FEBS Lett., 408 (1997) 232]. In the present paper we have studied whether this novel cationic liposome is prominent in nature to suppress cell growth of human cancer cells. Bcl-2 antisense phosphorothioate oligonucleotides (AS-ODNs) were complexed with the cationic liposomes with the derivative (I) and they were introduced into human cervix epithelial carcinoma cell lines HeLa, and mouse fibroblast NIH3T3 cells. An AS-ODNs targeting/bcl-2 gene induced probably apoptosis (including necrosis in some cases) in HeLa and NIH3T3 cells, however, nonsense oligonucleotides (NS-ODNs) corresponding to a scrambled-sequence control hardly induced apoptosis. Induction of apoptosis was much greater than that by commercially available DC-Chol liposomes. Fluorescence intensities of FITC-conjugated bcl-2 AS-ODNs were specifically found in the nucleus. The intensity of the AS-ODNs was mostly consistent with the amounts of Bcl-2 proteins observed by Western blot analysis in the target cells. The results showed the possibility that this new cationic cholesterol derivative might be very promising to be used for liposome-mediated gene targeting in vitro and in vivo.
巻・号 88(2)
ページ 313-20
公開日 2003-3-7
DOI 10.1016/s0168-3659(02)00484-4
PII S0168365902004844
PMID 12628337
MeSH Apoptosis / drug effects* Cations DNA, Neoplasm / biosynthesis DNA, Neoplasm / genetics Flow Cytometry Genes, Reporter / genetics Genes, bcl-2 / genetics* Humans Immunoblotting Liposomes Luciferases Microscopy, Confocal Neoplasms / pathology* Oligonucleotides, Antisense / administration & dosage* Oligonucleotides, Antisense / pharmacology* Tumor Cells, Cultured
IF 7.727
引用数 13
WOS 分野 CHEMISTRY, MULTIDISCIPLINARY PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞 HeLa(RCB0007) NIH3T3