| RRC ID |
41828
|
| 著者 |
Takaishi S, Sawada M, Seno H, Kayahara T, Morita-Fujisawa Y, Fukuzawa H, Chiba T.
|
| タイトル |
Growth promoting effect of thioredoxin on intestinal epithelial cells.
|
| ジャーナル |
Dig Dis Sci
|
| Abstract |
Paneth cells are located at the bases of intestinal crypts, and their cytoplasmic granules contain large amounts of zinc. We previously showed that administration of diphenylthiocarbazone (dithizone), a zinc chelater, to rats induced the selective death of Paneth cells. This was followed by a transient wave of epithelial cell proliferation in the entire crypts. In the study described here, we again applied this experimental model in an attempt to identify novel growth-promoting factors in the small intestine. Male Wistar rats were injected with dithizone and killed 6 hr later. Messenger RNAs (mRNAs) were extracted from the terminal ileum for the construction of a cDNA library. This library was then transfected into the human intestinal cell line Caco-2, and the cells that continued to grow in the medium containing only 1% FBS were cloned. One of the cDNA sequences identified from those transfection experiments was the full-length rat thioredoxin (TRX) gene. To confirm the growth-promoting effect of this cDNA, we transfected it into Caco-2 cells again. These clones proliferated in the medium containing only 1% FBS, while the control clones failed to show any growth. Transient oxidative stress exerted by the addition of oxidative reagents diamide and hydrogen peroxide partially suppressed the growth of TRX-transfected cells. Northern hybridization analysis revealed that TRX expression in rat ileum after dithizone treatment was altered in accordance with intestinal epithelial regeneration in the crypts. Single-cell RT-PCR also showed TRX mRNA expression in Paneth cells. These studies identify rat thioredoxin as a growth-promoting factor for intestinal epithelial cells.
|
| 巻・号 |
48(2)
|
| ページ |
379-85
|
| 公開日 |
2003-2-1
|
| DOI |
10.1023/a:1021952132241
|
| PMID |
12643619
|
| MeSH |
Animals
Base Sequence
Blotting, Northern
Caco-2 Cells / drug effects
Caco-2 Cells / metabolism
Cell Division / drug effects*
Culture Media
DNA, Complementary / analysis
Disease Models, Animal
Dithizone
Humans
Ileum / cytology
Ileum / drug effects
Male
Molecular Sequence Data
Paneth Cells / drug effects*
RNA, Messenger / analysis
Random Allocation
Rats
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
Thioredoxins / pharmacology*
|
| IF |
2.751
|
| 引用数 |
9
|
|
WOS 分野
|
GASTROENTEROLOGY & HEPATOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
CACO-2(RCB0988) |
