論文 - 詳細
| RRC ID | 41828 |
|---|---|
| 著者 | Takaishi S, Sawada M, Seno H, Kayahara T, Morita-Fujisawa Y, Fukuzawa H, Chiba T. |
| タイトル | Growth promoting effect of thioredoxin on intestinal epithelial cells. |
| ジャーナル | Dig Dis Sci |
| Abstract |
Paneth cells are located at the bases of intestinal crypts, and their cytoplasmic granules contain large amounts of zinc. We previously showed that administration of diphenylthiocarbazone (dithizone), a zinc chelater, to rats induced the selective death of Paneth cells. This was followed by a transient wave of epithelial cell proliferation in the entire crypts. In the study described here, we again applied this experimental model in an attempt to identify novel growth-promoting factors in the small intestine. Male Wistar rats were injected with dithizone and killed 6 hr later. Messenger RNAs (mRNAs) were extracted from the terminal ileum for the construction of a cDNA library. This library was then transfected into the human intestinal cell line Caco-2, and the cells that continued to grow in the medium containing only 1% FBS were cloned. One of the cDNA sequences identified from those transfection experiments was the full-length rat thioredoxin (TRX) gene. To confirm the growth-promoting effect of this cDNA, we transfected it into Caco-2 cells again. These clones proliferated in the medium containing only 1% FBS, while the control clones failed to show any growth. Transient oxidative stress exerted by the addition of oxidative reagents diamide and hydrogen peroxide partially suppressed the growth of TRX-transfected cells. Northern hybridization analysis revealed that TRX expression in rat ileum after dithizone treatment was altered in accordance with intestinal epithelial regeneration in the crypts. Single-cell RT-PCR also showed TRX mRNA expression in Paneth cells. These studies identify rat thioredoxin as a growth-promoting factor for intestinal epithelial cells. |
| 巻・号 | 48(2) |
| ページ | 379-85 |
| 公開日 | 2003-2-1 |
| DOI | 10.1023/a:1021952132241 |
| PMID | 12643619 |
| MeSH | Animals Base Sequence Blotting, Northern Caco-2 Cells / drug effects Caco-2 Cells / metabolism Cell Division / drug effects* Culture Media DNA, Complementary / analysis Disease Models, Animal Dithizone Humans Ileum / cytology Ileum / drug effects Male Molecular Sequence Data Paneth Cells / drug effects* RNA, Messenger / analysis Random Allocation Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction Sensitivity and Specificity Thioredoxins / pharmacology* |
| IF | 2.751 |
| 引用数 | 9 |
| WOS 分野 | GASTROENTEROLOGY & HEPATOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) |