論文 - 詳細
| RRC ID | 41840 |
|---|---|
| 著者 | Nagaoka T, Banskota AH, Tezuka Y, Midorikawa K, Matsushige K, Kadota S. |
| タイトル | Caffeic acid phenethyl ester (CAPE) analogues: potent nitric oxide inhibitors from the Netherlands propolis. |
| ジャーナル | Biol Pharm Bull |
| Abstract |
The MeOH and water extracts of the Netherlands propolis were tested for their inhibitory activity toward nitric oxide (NO) production in lipopolysaccharide (LPS)-activated murine macrophage-like J774.1 cells. Both of the extract possessed significant NO inhibitory activity with IC(50) values of 23.8 and 51.5 microg/ml, respectively. Then 13 phenolic compounds obtained from the MeOH extract showing stronger NO inhibition were examined on their NO inhibitory activities. Caffeic acid phenethyl ester (CAPE) analogues, i.e., benzyl caffeate, CAPE and cinnamyl caffeate, possessed most potent NO inhibitory activities with IC(50) values of 13.8, 7.64 and 9.53 microM, respectively, which were two- to four-fold stronger than the positive control N(G)-monomethyl-L-arginine (L-NMMA; IC(50), 32.9 microM). Further study on the synthetic analogues of CAPE revealed that both of 3-phenylpropyl caffeate (18; IC(50), 7.34 microM) and 4-phenylbutyl caffeate (19; IC(50), 6.77 microM) possessed stronger NO inhibitory activity than CAPE (10) and that elongation of alkyl side chain of alcoholic parts of caffeic acid esters enhance the NO inhibitory activity. In addition, it was found that CAPE analogues having longer carbon chain (>C(5)) in alcoholic part showed toxic effects toward J774.1 cells. This NO inhibitory effect may directly correlate with antiinflammatory properties of the Netherlands propolis. |
| 巻・号 | 26(4) |
| ページ | 487-91 |
| 公開日 | 2003-4-1 |
| DOI | 10.1248/bpb.26.487 |
| PMID | 12673030 |
| MeSH | Animals Caffeic Acids / chemistry* Caffeic Acids / isolation & purification Caffeic Acids / pharmacology* Cell Line Macrophages / drug effects Macrophages / physiology Mice Netherlands Nitric Oxide / antagonists & inhibitors* Nitric Oxide / biosynthesis Phenylethyl Alcohol / analogs & derivatives* Phenylethyl Alcohol / chemistry* Phenylethyl Alcohol / isolation & purification Phenylethyl Alcohol / pharmacology* Propolis / analogs & derivatives* Propolis / isolation & purification Propolis / pharmacology* |
| IF | 1.863 |
| 引用数 | 62 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | J774.1(RCB0434) |