RRC ID |
41849
|
著者 |
Yoshimura A, Gemma A, Hosoya Y, Komaki E, Hosomi Y, Okano T, Takenaka K, Matuda K, Seike M, Uematsu K, Hibino S, Shibuya M, Yamada T, Hirohashi S, Kudoh S.
|
タイトル |
Increased expression of the LGALS3 (galectin 3) gene in human non-small-cell lung cancer.
|
ジャーナル |
Genes Chromosomes Cancer
|
Abstract |
Patients with lung cancer have a poor prognosis because of the high metastatic potential of the neoplasm. Therefore, identifying new molecular targets for anti-metastatic therapy is very important. To identify novel key factors of tumor metastasis in lung cancer, we established the gene expression profiles of two adenocarcinoma cell line variants, PC9/f9 and PC9/f14, by use of genome-wide human cDNA microarray analysis and comparing these profiles with that of the parental cell line, PC9. The PC9/f9 and PC9/f14 cell lines were selected for analysis because of their high metastatic potential. We identified five genes in the highly metastatic cell lines that showed a significantly enhanced or reduced expression and that had not been reported to be involved in metastasis of lung cancer. One of the overexpressed genes that was identified encoded the beta-galactoside-binding protein LGALS3 (Galectin 3). LGALS3 has been reported to be overexpressed in a variety of human cancers, but not in lung cancer, and to be involved in tumor metastasis. We examined the expression of LGALS3 by use of real-time quantitative reverse transcription-polymerase chain reaction in 38 lung cancer cell lines and in tumor tissue obtained by thoracoscopic biopsy. A population (10/30) of the non-small-cell lung cancers examined was found to overexpress the LGALS3 gene at levels three times higher than those of normal epithelial cells. In contrast, all small-cell lung cancers either failed to express the gene or expressed it at a very low level. The mean of the relative expression of the LGALS3 gene in non-small-cell lung cancer (3.065 +/- 3.976) was significantly higher than those of small-cell lung cancer (0.02 +/- 0.03) (P < 0.025). This is the first report of alterations of LGALS3 gene expression in lung cancer. These results, together with the previous reports on Galectin 3 function, suggest that Galectin 3 may play a role in the process of metastasis in non-small-cell lung cancer that overexpresses Galectin 3, but not in small-cell cancer. Accordingly, LGALS3 may be a phenotypic marker that excludes small-cell lung cancer and may represent a novel target molecule in non-small-cell lung cancer therapy.
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巻・号 |
37(2)
|
ページ |
159-64
|
公開日 |
2003-6-1
|
DOI |
10.1002/gcc.10205
|
PMID |
12696064
|
MeSH |
Adenocarcinoma / genetics
Adenocarcinoma / pathology
Animals
Carcinoma, Non-Small-Cell Lung / genetics*
Carcinoma, Non-Small-Cell Lung / pathology
Carcinoma, Non-Small-Cell Lung / secondary
Galectin 3 / biosynthesis*
Galectin 3 / genetics*
Gene Expression Profiling / methods*
Gene Expression Regulation, Neoplastic / genetics*
Genes, Neoplasm / genetics
Humans
Lung Neoplasms / genetics*
Lung Neoplasms / pathology
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Neoplasm Transplantation / methods
Oligonucleotide Array Sequence Analysis / methods
Tumor Cells, Cultured
Up-Regulation / genetics*
|
IF |
3.444
|
引用数 |
27
|
WOS 分野
|
GENETICS & HEREDITY
ONCOLOGY
|
リソース情報 |
ヒト・動物細胞 |
RERF-LC-KJ(RCB1313)
LC-2/ad(RCB0440)
SQ-5(RCB0110)
LC-1/sq(RCB0455)
LC-1F(RCB0439)
RERF-LC-AI(RCB0444)
Lu-130(RCB0465)
Lu-139(RCB0469)
Lu-165(RCB1184)
MS-1(RCB0725) |