論文 - 詳細
| RRC ID | 41849 |
|---|---|
| 著者 | Yoshimura A, Gemma A, Hosoya Y, Komaki E, Hosomi Y, Okano T, Takenaka K, Matuda K, Seike M, Uematsu K, Hibino S, Shibuya M, Yamada T, Hirohashi S, Kudoh S. |
| タイトル | Increased expression of the LGALS3 (galectin 3) gene in human non-small-cell lung cancer. |
| ジャーナル | Genes Chromosomes Cancer |
| Abstract |
Patients with lung cancer have a poor prognosis because of the high metastatic potential of the neoplasm. Therefore, identifying new molecular targets for anti-metastatic therapy is very important. To identify novel key factors of tumor metastasis in lung cancer, we established the gene expression profiles of two adenocarcinoma cell line variants, PC9/f9 and PC9/f14, by use of genome-wide human cDNA microarray analysis and comparing these profiles with that of the parental cell line, PC9. The PC9/f9 and PC9/f14 cell lines were selected for analysis because of their high metastatic potential. We identified five genes in the highly metastatic cell lines that showed a significantly enhanced or reduced expression and that had not been reported to be involved in metastasis of lung cancer. One of the overexpressed genes that was identified encoded the beta-galactoside-binding protein LGALS3 (Galectin 3). LGALS3 has been reported to be overexpressed in a variety of human cancers, but not in lung cancer, and to be involved in tumor metastasis. We examined the expression of LGALS3 by use of real-time quantitative reverse transcription-polymerase chain reaction in 38 lung cancer cell lines and in tumor tissue obtained by thoracoscopic biopsy. A population (10/30) of the non-small-cell lung cancers examined was found to overexpress the LGALS3 gene at levels three times higher than those of normal epithelial cells. In contrast, all small-cell lung cancers either failed to express the gene or expressed it at a very low level. The mean of the relative expression of the LGALS3 gene in non-small-cell lung cancer (3.065 +/- 3.976) was significantly higher than those of small-cell lung cancer (0.02 +/- 0.03) (P < 0.025). This is the first report of alterations of LGALS3 gene expression in lung cancer. These results, together with the previous reports on Galectin 3 function, suggest that Galectin 3 may play a role in the process of metastasis in non-small-cell lung cancer that overexpresses Galectin 3, but not in small-cell cancer. Accordingly, LGALS3 may be a phenotypic marker that excludes small-cell lung cancer and may represent a novel target molecule in non-small-cell lung cancer therapy. |
| 巻・号 | 37(2) |
| ページ | 159-64 |
| 公開日 | 2003-6-1 |
| DOI | 10.1002/gcc.10205 |
| PMID | 12696064 |
| MeSH | Adenocarcinoma / genetics Adenocarcinoma / pathology Animals Carcinoma, Non-Small-Cell Lung / genetics* Carcinoma, Non-Small-Cell Lung / pathology Carcinoma, Non-Small-Cell Lung / secondary Galectin 3 / biosynthesis* Galectin 3 / genetics* Gene Expression Profiling / methods* Gene Expression Regulation, Neoplastic / genetics* Genes, Neoplasm / genetics Humans Lung Neoplasms / genetics* Lung Neoplasms / pathology Male Mice Mice, Inbred BALB C Mice, Nude Neoplasm Transplantation / methods Oligonucleotide Array Sequence Analysis / methods Tumor Cells, Cultured Up-Regulation / genetics* |
| IF | 3.444 |
| 引用数 | 27 |
| WOS 分野 | GENETICS & HEREDITY ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | RERF-LC-KJ(RCB1313) LC-2/ad(RCB0440) SQ-5(RCB0110) LC-1/sq(RCB0455) LC-1F(RCB0439) RERF-LC-AI(RCB0444) Lu-130(RCB0465) Lu-139(RCB0469) Lu-165(RCB1184) MS-1(RCB0725) |