Reference - Detail
| RRC ID | 41853 |
|---|---|
| Author | Kitamura H, Matsushita Y, Iwanaga T, Mori K, Kanehira K, Fujikura D, Morimatsu M, Saito M. |
| Title | Bacterial lipopolysaccharide-induced expression of the IkappaB protein MAIL in B-lymphocytes and macrophages. |
| Journal | Arch Histol Cytol |
| Abstract |
Molecule possessing ankyrin-repeats induced by lipopolysaccharide (MAIL), a recently cloned nuclear IkappaB protein induced by lipopolysaccharide (LPS) stimulation in lymphoid organs, is involved in the regulation of inflammatory responses. The present in situ hybridization and immunohistochemical analyses revealed the distinct expression of the MAIL mRNA and protein in B-lymphocytes of the white pulp of the spleen and cortical lymphoid follicles of lymph nodes in LPS-injected mice. MAIL signals were also localized in F4/80-positive macrophages in these organs. LPS clearly induced MAIL expression in cultured B-lymphocytes and monocytes/macrophages, but only faintly so in T-lymphocytes, fibroblasts, and endothelial cells. MAIL was also induced by inflammatory cytokines such as interleukin-1 and -6, and tumor necrosis factor in cultured cells. Northern blot, Western blot, and in situ hybridization analyses showed that the major expression product of the Mail gene was a long splicing variant (MAIL-L) rather than a short one, both in lymphoid organs and cultured cells. These results collectively indicate that LPS induces MAIL-L predominantly in B-lymphocytes and macrophages. |
| Volume | 66(1) |
| Pages | 53-62 |
| Published | 2003-3-1 |
| DOI | 10.1679/aohc.66.53 |
| PMID | 12703554 |
| MeSH | Adaptor Proteins, Signal Transducing Animals B-Lymphocytes / cytology B-Lymphocytes / drug effects B-Lymphocytes / physiology* Blotting, Western Cell Line Gene Expression / drug effects Gene Expression / immunology Immunohistochemistry In Situ Hybridization Lipopolysaccharides / pharmacology* Lymphoid Tissue / cytology Macrophages, Peritoneal / cytology Macrophages, Peritoneal / drug effects Macrophages, Peritoneal / physiology* Male Mice Mice, Inbred C57BL Nuclear Proteins / genetics* Nuclear Proteins / metabolism RNA, Messenger / analysis T-Lymphocytes / cytology T-Lymphocytes / drug effects T-Lymphocytes / physiology |
| Times Cited | 13 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 6 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | CTLL-2(RCB0637) 3T3 Swiss Albino(RCB1642) |