論文 - 詳細
| RRC ID | 41863 |
|---|---|
| 著者 | Shin SY, Yokoyama T, Takenouchi T, Munekata E. |
| タイトル | The chemical synthesis and binding affinity to the EGF receptor of the EGF-like domain of heparin-binding EGF-like growth factor (HB-EGF). |
| ジャーナル | J Pept Sci |
| Abstract |
Heparin-binding EGF-like growth factor (HB-EGF), which belongs to the EGF-family of growth factors, was isolated from the conditioned medium of macrophage-like cells. To investigate the effect of N- and C-terminal residues of the EGF-like domain of HB-EGF in the binding affinity to the EGF receptor on A431 cell. We synthesized HB-EGF(44-86) corresponding to the EGF-like domain of HB-EGF and its N- or C-terminal truncated peptides. Thermolytic digestion demonstrated three disulfide bond pairings of the EGF-like domain in HB-EGF is consistent with that of human-EGF and human-TGF-alpha. HB-EGF(44-86) showed high binding affinity to EGF-receptor, like human-EGF. The truncation of the C-terminal Leu86 residue from HB-EGF(44-86), HB-EGF(45-86) or HB-EGF(46-86) caused a drastic reduction in the binding affinity to the EGF receptor. These results suggest that the EGF-like domain of HB-EGF plays an important role in the binding to the EGF receptor, and its C-terminal Leu86 residue is necessary for binding with the EGF-receptor. In addition, the deletion of the two N-terminal residues (Asp44-Pro45) from HB-EGF(44-86) caused a 10-fold decrease in relative binding affinity to the EGF receptor. This indicates that the two N-terminal residues of the EGF-like domain of HB-EGF are necessary for its optimal binding affinity to the EGF receptor. |
| 巻・号 | 9(4) |
| ページ | 244-50 |
| 公開日 | 2003-4-1 |
| DOI | 10.1002/psc.450 |
| PMID | 12725245 |
| MeSH | Amino Acid Sequence Binding Sites Cell Line, Tumor Epidermal Growth Factor / chemical synthesis Epidermal Growth Factor / chemistry* Epidermal Growth Factor / metabolism* ErbB Receptors / metabolism* Heparin-binding EGF-like Growth Factor Humans Intercellular Signaling Peptides and Proteins Peptide Fragments / chemical synthesis Protein Binding Protein Structure, Tertiary Transforming Growth Factor alpha / chemistry |
| IF | 1.877 |
| 引用数 | 4 |
| WOS 分野 | CHEMISTRY, ANALYTICAL BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A431(RCB0202) |