| RRC ID |
41867
|
| Author |
Miura Y, Kozuki Y, Yagasaki K.
|
| Title |
Potentiation of invasive activity of hepatoma cells by reactive oxygen species is mediated by autocrine/paracrine loop of hepatocyte growth factor.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
We have already reported that reactive oxygen species (ROS) promote rat ascites hepatoma cell invasion beneath mesentery-derived mesothelial cell monolayer. To investigate the mechanism for this, we examined the involvement of motility factors, particularly hepatocyte growth factor (HGF). Rat ascites hepatoma cell line of AH109A expressed HGF and c-Met mRNAs. Treatment with ROS augmented amounts of HGF mRNA in AH109A and HGF concentration in the medium. ROS also induced HGF gene expression in mesothelial cells. Exogenously added HGF enhanced invasive activity of AH109A cells, but exerted no effect on proliferation. AH109A cells pretreated with ROS showed an increased invasive activity, which was cancelled by simultaneous pretreatment with anti-HGF antibody. These results suggest that the invasive activity of AH109A is mediated by the autocrine and paracrine pathways of HGF, and ROS potentiate invasive activity by inducing gene expression of HGF in AH109A and mesothelial cells.
|
| Volume |
305(1)
|
| Pages |
160-5
|
| Published |
2003-5-23
|
| DOI |
10.1016/s0006-291x(03)00725-3
|
| PII |
S0006291X03007253
|
| PMID |
12732211
|
| MeSH |
Animals
Autocrine Communication
Carcinoma, Hepatocellular / metabolism
Carcinoma, Hepatocellular / pathology
Carcinoma, Hepatocellular / physiopathology*
Cell Division / drug effects
Cell Movement* / drug effects
Coculture Techniques
Drug Synergism
Epithelial Cells / drug effects
Epithelial Cells / metabolism
Hepatocyte Growth Factor / genetics
Hepatocyte Growth Factor / pharmacology
Hepatocyte Growth Factor / physiology*
Liver Neoplasms / metabolism
Liver Neoplasms / pathology
Liver Neoplasms / physiopathology*
Male
Neoplasm Invasiveness
Paracrine Communication
RNA, Messenger / biosynthesis
Rats
Reactive Oxygen Species / pharmacology*
Tumor Cells, Cultured
|
| IF |
2.985
|
| Times Cited |
32
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
L929 |
