論文 - 詳細
| RRC ID | 41881 |
|---|---|
| 著者 | Reyes CD, García AJ. |
| タイトル | Engineering integrin-specific surfaces with a triple-helical collagen-mimetic peptide. |
| ジャーナル | J Biomed Mater Res A |
| Abstract |
Integrin-mediated cell adhesion to extracellular matrix proteins anchors cells and triggers signals that direct cell function. The integrin alpha(2)beta(1) recognizes the glycine-phenylalanine-hydroxyproline-glycine-glutamate-arginine (GFOGER) motif in residues 502-507 of the alpha(1)(I) chain of type I collagen. Integrin recognition is entirely dependent on the triple-helical conformation of the ligand similar to that of native collagen. This study focuses on engineering alpha(2)beta(1)-specific bioadhesive surfaces by immobilizing a triple-helical collagen-mimetic peptide incorporating the GFOGER binding sequence onto model nonadhesive substrates. Circular dichroism spectroscopy verified that this peptide adopts a stable triple-helical conformation in solution. Passively adsorbed GFOGER-peptide exhibited dose-dependent HT1080 cell adhesion and spreading comparable to that observed on type I collagen. Subsequent antibody blocking conditions verified the involvement of integrin alpha(2)beta(1) in these adhesion events. Focal adhesion formation was observed by immunofluorescent staining for alpha(2)beta(1) and vinculin on MC3T3-E1 cells. Model functionalized surfaces then were engineered using three complementary peptide-tethering schemes. These peptide-functionalized substrates supported alpha(2)beta(1)-mediated cell adhesion and focal adhesion assembly. Our results suggest that this peptide is active in an immobilized conformation and may be applied as a surface modification agent to promote alpha(2)beta(1)-specific cell adhesion. Engineering surfaces that specifically target certain integrin-ligand interactions and signaling cascades provides a biomolecular strategy for optimizing cellular responses in biomaterials and tissue engineering applications. |
| 巻・号 | 65(4) |
| ページ | 511-23 |
| 公開日 | 2003-6-15 |
| DOI | 10.1002/jbm.a.10550 |
| PMID | 12761842 |
| MeSH | 3T3 Cells Amino Acid Sequence Animals Antibodies Avidin Binding Sites Biocompatible Materials / chemical synthesis Biocompatible Materials / chemistry* Buffers Cell Adhesion Cell Line Circular Dichroism Collagen / chemistry* Focal Adhesions Humans Integrin alpha2beta1 / metabolism* Materials Testing Mice Molecular Mimicry Oligopeptides / chemical synthesis Oligopeptides / chemistry* Protein Conformation Protein Structure, Quaternary Tissue Engineering / methods* |
| IF | 3.525 |
| 引用数 | 124 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |