論文 - 詳細
| RRC ID | 41889 |
|---|---|
| 著者 | Fratzl-Zelman N, Glantschnig H, Rumpler M, Nader A, Ellinger A, Varga F. |
| タイトル | The expression of matrix metalloproteinase-13 and osteocalcin in mouse osteoblasts is related to osteoblastic differentiation and is modulated by 1,25-dihydroxyvitamin D3 and thyroid hormones. |
| ジャーナル | Cell Biol Int |
| Abstract |
Matrix metalloproteinase-13 (MMP-13), is a key protein of bone matrix degradation, and is highly expressed by osteoblasts. We used the osteoblast-like MC3T3-E1 cell line and compared the stimulatory effects of the bone resorptive agents 1,25-dihydroxyvitamin D3 (1,25-(OH)(2)D(3)) 3,3',5-triido-L-thyronine (T3) on the expression of MMP-13 mRNA. We showed that the stimulatory effects were time and dose dependent, and were also transduced to the protein level, with 1,25-(OH)(2)D(3)being more potent.MMP-13 expression in different mouse cells and its localization within developing bone from the onset of osteogenesis were also investigated. 1,25-(OH)(2)D(3)- and T3-regulated osteocalcin (Osc) expression in mouse osteoblasts was compared to hormonal effects on MMP-13 expression and activity. Here we show divergent and common roles of 1,25-(OH)(2)D(3)and T3 action on the expression of these marker proteins, depending on the stage of cell differentiation. In addition, we propose a role for MMP-13 in the bone collar of developing long bones. The results could help to more precisely characterize hormonal regulation in the developmental sequence of osteoblasts. |
| 巻・号 | 27(6) |
| ページ | 459-68 |
| 公開日 | 2003-1-1 |
| DOI | 10.1016/s1065-6995(03)00037-4 |
| PII | S1065699503000374 |
| PMID | 12798773 |
| MeSH | Animals Animals, Newborn Bone Matrix / drug effects Bone Matrix / enzymology Bone Resorption / chemically induced Bone Resorption / enzymology Calcitriol / metabolism* Calcitriol / pharmacology Cell Differentiation / drug effects Cell Differentiation / physiology* Collagenases / biosynthesis Collagenases / genetics* Dose-Response Relationship, Drug Fetus Gene Expression Regulation, Enzymologic / drug effects Gene Expression Regulation, Enzymologic / physiology Matrix Metalloproteinase 13 Mice Mice, Inbred C3H NIH 3T3 Cells Osteoblasts / cytology Osteoblasts / drug effects Osteoblasts / enzymology* Osteocalcin / metabolism* Osteogenesis / drug effects Osteogenesis / physiology RNA, Messenger / drug effects RNA, Messenger / metabolism Radius / drug effects Radius / enzymology Radius / growth & development Reaction Time / drug effects Reaction Time / physiology Skull / drug effects Skull / enzymology Skull / growth & development Triiodothyronine / metabolism* Triiodothyronine / pharmacology Ulna / drug effects Ulna / enzymology Ulna / growth & development |
| IF | 2.571 |
| 引用数 | 32 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | ST2(RCB0224) |